Cell Death Antibodies 2
Anti-RIPK1 Antibody (CAB19580)
- SKU:
- CAB19580
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
Antibody Name: | Anti-RIPK1 Antibody |
Antibody SKU: | CAB19580 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IP |
Reactivity: | Human, Mouse |
Host Species: | Rabbit |
Immunogen: | A synthesized peptide derived from human RIPK1. |
Application: | WB IP |
Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:200 |
Reactivity: | Human, Mouse |
Positive Samples: | Raji, HeLa |
Immunogen: | A synthesized peptide derived from human RIPK1. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Gene ID: | 8737 |
Uniprot: | Q13546 |
Cellular Location: | Cell membrane, Cytoplasm |
Calculated MW: | 75kDa |
Observed MW: | 75KDa |
Synonyms: | RIP, RIP-1, RIP1, RIPK1 |
Background: |
UniProt Protein Function: | RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family Chromosomal Location of Human Ortholog: 6p25.2 Cellular Component: mitochondrion; cytoplasm; cytosol; receptor complex; lipid raft Molecular Function:identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; protein complex binding; ATP binding; protein kinase activity Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; positive regulation of apoptosis; apoptosis; MyD88-independent toll-like receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; tumor necrosis factor-mediated signaling pathway; cellular protein catabolic process; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; protein homooligomerization |
NCBI Summary: | This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017] |
UniProt Code: | Q13546 |
NCBI GenInfo Identifier: | 57242761 |
NCBI Gene ID: | 8737 |
NCBI Accession: | NP_003795.2 |
UniProt Secondary Accession: | Q13546,Q13180, Q59H33, A0AV89, B2RAG1, B4E3F9, |
UniProt Related Accession: | Q13546 |
Molecular Weight: | 75931 MW |
NCBI Full Name: | receptor-interacting serine/threonine-protein kinase 1 isoform 1 |
NCBI Synonym Full Names: | receptor interacting serine/threonine kinase 1 |
NCBI Official Symbol: | RIPK1Ā Ā |
NCBI Official Synonym Symbols: | RIP; RIP1; RIP-1Ā Ā |
NCBI Protein Information: | receptor-interacting serine/threonine-protein kinase 1 |
UniProt Protein Name: | Receptor-interacting serine/threonine-protein kinase 1 |
UniProt Synonym Protein Names: | Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP |
Protein Family: | Receptor-interacting serine/threonine-protein kinase |
UniProt Gene Name: | RIPK1Ā Ā |
UniProt Entry Name: | RIPK1_HUMAN |