KO Validated Antibodies 1

Anti-RING1B/RNF2 Antibody (CAB18076)[KO Validated]

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SKU:
CAB18076
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Applications:
IP
Applications:
ChIP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RING1B/RNF2 Antibody [KO Validated]
Antibody SKU:CAB18076
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP ChIP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1).
Application:WB IHC IF IP ChIP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSQA VQTN GTQP LSKT WELS LYEL QRTP QEAI TDGL EIVV SPRS LHSE LMCP ICLD MLKN TMTT KECL HRFC ADCI ITAL RSGN KECP TCRK KLVS KRSL RPDP NFDA LISK IYPS RDEY EAHQ ERVL ARIN KHNN QQAL SHSI EEGL KIQA MNRL QRGK KQQI ENGS GAED NGDS SHCS NAST HSNQ EAGP SNKR TKTS DDSG LELD NNNA AMAI DPVM DGAS EIEL VFRP HPTL MEKD DSAQ TRYI KTSG NATV DHLS KYLA VRLA LEEL RSKG ESNQ MNLD TASE KQYT IYIA TASG QFTV LNGS FSLE LVSE KYWK VNKP MELY YAPT KEHK
Gene ID:6045
Uniprot:Q99496
Cellular Location:Chromosome, Nucleus
Calculated MW:29kDa/37kDa
Observed MW:38kDa
Western blot - [KO Validated] RING1B/RNF2 Rabbit pAb Western blot analysis of extracts from normal (control) and RING1B/RNF2 knockout (KO) HeLa cells, using RING1B/RNF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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