Cell Biology Antibodies 4
Anti-RIN1 Antibody (CAB13791)
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- SKU:
- CAB13791
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RIN1 Antibody |
| Antibody SKU: | CAB13791 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RIN1 (NP_004283.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RIN1 (NP_004283.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MESP GESG AGSP GAPS PSSF TTGH LARE KPAQ DPLY DVPN ASGG QAGG PQRP GRVV SLRE RLLL TRPV WLQL QANA AAAL HMLR TEPP GTFL VRKS NTRQ CQAL CMRL PEAS GPSF VSSH YILE SPGG VSLE GSEL MFPD LVQL ICAY CHTR DILL LPLQ LPRA IHHA ATHK ELEA ISHL GIEF WSSS LNIK AQRG PAGG PVLP QLKA RSPQ ELDQ GTGA ALCF FNPL FPGD LGPT KREK FKRS FKVR VSTE TSSP LSPP AVPP PPVP VLPG AVPS QTER |
| Gene ID: | 9610 |
| Uniprot: | Q13671 |
| Cellular Location: | Cytoplasm, Membrane, cytoskeleton |
| Calculated MW: | 77kDa/84kDa |
| Observed MW: | 84kDa |
| Synonyms: | RIN1 |
| Background: |
| UniProt Protein Function: | Rin1: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis. Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351. Expressed in all tissues examined with high levels in brain, placenta and pancreas. Belongs to the RIN (Ras interaction/interference) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, Rab Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: cytoplasm; plasma membrane Molecular Function:protein binding |
| UniProt Code: | Q13671 |
| NCBI GenInfo Identifier: | 116242760 |
| NCBI Gene ID: | 9610 |
| NCBI Accession: | Q13671.4 |
| UniProt Secondary Accession: | Q13671,O15010, Q00427, Q96CC8, |
| UniProt Related Accession: | Q13671 |
| Molecular Weight: | 77,500 Da |
| NCBI Full Name: | Ras and Rab interactor 1 |
| NCBI Synonym Full Names: | Ras and Rab interactor 1 |
| NCBI Official Symbol: | RIN1 |
| NCBI Protein Information: | ras and Rab interactor 1 |
| UniProt Protein Name: | Ras and Rab interactor 1 |
| UniProt Synonym Protein Names: | Ras inhibitor JC99; Ras interaction/interference protein 1 |
| Protein Family: | RuvB-like protein |
| UniProt Gene Name: | RIN1 |
| UniProt Entry Name: | RIN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RIN1 antibody (CAB13791) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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