Cell Cycle Antibodies 1

Anti-RIF1 Antibody (CAB15167)

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SKU:
CAB15167
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-RIF1 Antibody
Antibody SKU:CAB15167
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2173-2472 of human RIF1 (NP_060621.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG, HeLa, 293T, LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2173-2472 of human RIF1 (NP_060621.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PLAS PSTS ILKR GLKR SQED EISS PVNK VRRV SFAD PIYQ AGLA DDID RRCS IVRS HSSN SSPI GKSV KTSP TTQS KHNT TSAK GFLS PGSR SPKF KSSK KCLI SEMA KESI PCPT ESVY PPLV NCVA PVDI ILPQ ITSN MWAR GLGQ LIRA KNIK TIGD LSTL TASE IKTL PIRS PKVS NVKK ALRI YHEQ QVKT RGLE EIPV FDIS EKTV NGIE NKSL SPDE ERLV SDII DPVA LEIP LSKN LLAQ ISAL ALQL DSED LHNY SGSQ LFEM HEKL SCMA NSVI KNLQ SRWR SPSH ENSI
Gene ID:55183
Uniprot:Q5UIP0
Cellular Location:Chromosome, Cytoplasm, Nucleus, cytoskeleton, spindle, telomere
Calculated MW:271kDa/274kDa
Observed MW:350kDa
Synonyms:RIF1
Background:This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:RIF1: Required for checkpoint mediated arrest of cell cycle progression in response to DNA damage during S-phase (the intra-S- phase checkpoint). This checkpoint requires activation of at least 2 parallel pathways by the ATM kinase: one involves the MRN (MRE11A-RAD50-NBS1) complex, while the second requires CHEK2. RIF1 seems to act independently of both these pathways. Seems to play no role in either the G1/S or G2/M DNA damage checkpoints. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA repair, damage

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: condensed chromosome; nucleoplasm; nucleus; plasma membrane; spindle midzone

Biological Process: double-strand break repair via nonhomologous end joining; telomere maintenance in response to DNA damage

NCBI Summary:This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
UniProt Code:Q5UIP0
NCBI GenInfo Identifier:56676335
NCBI Gene ID:55183
NCBI Accession:NP_060621.3
UniProt Secondary Accession:Q5UIP0,Q5H9R3, Q5UIP2, Q66YK6, Q6PRU2, Q8TE94, Q99772 Q9H830, Q9H9B9, Q9NVP5, A6NC27, C9JBR1,
UniProt Related Accession:Q5UIP0
Molecular Weight:271,689 Da
NCBI Full Name:telomere-associated protein RIF1 isoform 1
NCBI Synonym Full Names:replication timing regulatory factor 1
NCBI Official Symbol:RIF1  
NCBI Protein Information:telomere-associated protein RIF1
UniProt Protein Name:Telomere-associated protein RIF1
UniProt Synonym Protein Names:Rap1-interacting factor 1 homolog
Protein Family:Telomere-associated protein
UniProt Gene Name:RIF1  
UniProt Entry Name:RIF1_HUMAN
Anti-RIF1 Antibody (CAB15167)Western blot analysis of extracts of various cell lines, using RIF1 antibody (CAB15167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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