Immunology Antibodies 2

Anti-RICTOR Antibody (CAB6131)

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SKU:
CAB6131
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-RICTOR Antibody
Antibody SKU:CAB6131
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human RICTOR (NP_689969.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human RICTOR (NP_689969.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAI GRGR SLKN LRVR GRND SGEE NVPL DLTR EPSD NLRE ILQN VARL QGVS NMRK LGHL NNFT KLLC DIGH SEEK LGFH YEDI IICL RLAL LNEA KEVR AAGL RALR YLIQ DSSI LQKV LKLK VDYL IARC IDIQ QSNE VERT QALR LVRK MITV NASL FPSS VTNS LIAV GNDG LQER DRMV RACI AIIC ELAL QNPE VVAL RGGL NTIL KNVI DCQL SRIN EALI TTIL HLLN HPKT RQYV R
Gene ID:253260
Uniprot:Q6R327
Cellular Location:
Calculated MW:28kDa/192kDa/195kDa
Observed MW:192kDa
Synonyms:RICTOR, AVO3, PIA, hAVO3
Background:
UniProt Protein Function:RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.
UniProt Protein Details:

Protein type:Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: cytosol; TORC2 complex

Molecular Function:enzyme activator activity; protein binding; protein kinase binding

Biological Process: actin cytoskeleton reorganization; embryonic development; establishment and/or maintenance of actin cytoskeleton polarity; establishment of cell polarity; peptidyl-serine phosphorylation; phosphoinositide-mediated signaling; positive regulation of protein kinase B signaling cascade; positive regulation of TOR signaling pathway; regulation of actin cytoskeleton organization and biogenesis; regulation of phosphorylation; regulation of protein kinase B signaling cascade; T cell costimulation

NCBI Summary:RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]
UniProt Code:Q6R327
NCBI GenInfo Identifier:74710068
NCBI Gene ID:253260
NCBI Accession:Q6R327.1
UniProt Secondary Accession:Q6R327,Q68DT5, Q86UB7, Q8N3A0, Q8NCM6, B2RNX0, B7ZMF7
UniProt Related Accession:Q6R327
Molecular Weight:195,011 Da
NCBI Full Name:Rapamycin-insensitive companion of mTOR
NCBI Synonym Full Names:RPTOR independent companion of MTOR complex 2
NCBI Official Symbol:RICTOR  
NCBI Official Synonym Symbols:PIA; AVO3; hAVO3  
NCBI Protein Information:rapamycin-insensitive companion of mTOR
UniProt Protein Name:Rapamycin-insensitive companion of mTOR
UniProt Synonym Protein Names:AVO3 homolog; hAVO3
Protein Family:Rapamycin-insensitive companion
UniProt Gene Name:RICTOR  
UniProt Entry Name:RICTR_HUMAN
Anti-RICTOR Antibody (CAB6131)Western blot analysis of extracts of various cell lines, using RICTOR antibody (CAB6131) at 1:300 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
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