Cell Biology Antibodies 1
Anti-RIC8A Antibody (CAB10393)
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- SKU:
- CAB10393
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RIC8A Antibody |
| Antibody SKU: | CAB10393 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-531 of human RIC8A (NP_001273063.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, HeLa, HepG2, 293T, DU145, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 362-531 of human RIC8A (NP_001273063.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DVRT RPEV GEML RNKL VRLM THLD TDVK RVAA EFLF VLCS ESVP RFIK YTGY GNAA GLLA ARGL MAGG RPEG QYSE DEDT DTDE YKEA KASI NPVT GRVE EKPP NPME GMTE EQKE HEAM KLVT MFDK LSRN RVIQ PMGM SPRG HLTS LQDA MCET MEQQ LSSD PDSD PD |
| Gene ID: | 60626 |
| Uniprot: | Q9NPQ8 |
| Cellular Location: | Cell membrane, Cytoplasm |
| Calculated MW: | 58kDa/59kDa/60kDa |
| Observed MW: | 60kDa |
| Synonyms: | RIC8A, RIC8 |
| Background: |
| UniProt Protein Function: | RIC8A: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. Belongs to the synembryn family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, misc. Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: cytoplasm; plasma membrane Molecular Function:G-protein alpha-subunit binding; GTPase activator activity; guanyl-nucleotide exchange factor activity; protein binding Biological Process: G-protein coupled receptor protein signaling pathway |
| UniProt Code: | Q9NPQ8 |
| NCBI GenInfo Identifier: | 308153562 |
| NCBI Gene ID: | 60626 |
| NCBI Accession: | Q9NPQ8.3 |
| UniProt Secondary Accession: | Q9NPQ8,Q0P508, Q2T9J1, Q7Z352, Q96EZ1, Q96SZ2, Q9H064 Q9H5H3, Q9H9E7, |
| UniProt Related Accession: | Q9NPQ8 |
| Molecular Weight: | 59,612 Da |
| NCBI Full Name: | Synembryn-A |
| NCBI Synonym Full Names: | RIC8 guanine nucleotide exchange factor A |
| NCBI Official Symbol: | RIC8A |
| NCBI Official Synonym Symbols: | RIC8 |
| NCBI Protein Information: | synembryn-A |
| UniProt Protein Name: | Synembryn-A |
| UniProt Synonym Protein Names: | Protein Ric-8A |
| Protein Family: | Synembryn |
| UniProt Gene Name: | RIC8A |
| UniProt Entry Name: | RIC8A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RIC8A antibody (CAB10393) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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