Cell Biology Antibodies 12

Anti-Ribonuclease H1 Antibody (CAB9116)

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SKU:
CAB9116
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Ribonuclease H1 Antibody
Antibody SKU:CAB9116
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human RNASEH1 (NP_002927.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse testis, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human RNASEH1 (NP_002927.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSWL LFLA HRVA LAAL PCRR GSRG FGMF YAVR RGRK TGVF LTWN ECRA QVDR FPAA RFKK FATE DEAW AFVR KSAS PEVS EGHE NQHG QESE AKAS KRLR EPLD GDGH ESAE PYAK HMKP SVEP APPV SRDT FSYM GDFV VVYT DGCC SSNG RRRP RAGI GVYW GPGH PLNV GIRL PGRQ TNQR AEIH AACK AIEQ AKTQ NINK LVLY TDSM FTIN GITN WVQG WKKN GWKT SAGK EVIN KEDF VALE RLTQ GMDI QWMH VPGH SGFI GNEE ADRL AREG AKQS ED
Gene ID:246243
Uniprot:O60930
Cellular Location:Cytoplasm
Calculated MW:32kDa
Observed MW:37kDa
Synonyms:RNASEH1, H1RNA, PEOB2, RNH1
Background:This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes; two of them are at different locations of chromosome 17 and one of them is on chromosome 1q32.2.
NCBI Summary:This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017]
UniProt Code:O60930
NCBI GenInfo Identifier:21359816
NCBI Gene ID:246243
NCBI Accession:NP_002927
UniProt Related Accession:O60930
Molecular Weight:Predicted Molecular Mass: 62.1kDaAccurate Molecular Mass: 62kDa
NCBI Full Name:ribonuclease H1 isoform 1
NCBI Synonym Full Names:ribonuclease H1
NCBI Official Symbol:RNASEH1  
NCBI Official Synonym Symbols:RNH1; H1RNA; PEOB2  
NCBI Protein Information:ribonuclease H1
UniProt Protein Name:Ribonuclease H1
UniProt Synonym Protein Names:Ribonuclease H type II
Protein Family:Ribonuclease
UniProt Gene Name:RNASEH1  
UniProt Entry Name:RNH1_HUMAN
Anti-Ribonuclease H1 Polyclonal Antibody (CAB9116)Western blot analysis of extracts of various cell lines, using RNASEH1 antibody (CAB9116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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