Cell Biology Antibodies 12
Anti-Ribonuclease 3 Antibody (CAB8336)
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- SKU:
- CAB8336
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Ribonuclease 3 Antibody |
| Antibody SKU: | CAB8336 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DROSHA (NP_037367.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DROSHA (NP_037367.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MMQG NTCH RMSF HPGR GCPR GRGG HGAR PSAP SFRP QNLR LLHP QQPP VQYQ YEPP SAPS TTFS NSPA PNFL PPRP DFVP FPPP MPPS AQGP LPPC PIRP |
| Gene ID: | 29102 |
| Uniprot: | Q9NRR4 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 138kDa/151kDa/155kDa/159kDa |
| Observed MW: | 159KDa |
| Synonyms: | DROSHA, ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN |
| Background: | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. |
| UniProt Protein Function: | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. |
| NCBI Summary: | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016] |
| UniProt Code: | Q9NRR4 |
| NCBI GenInfo Identifier: | 155030236 |
| NCBI Gene ID: | 29102 |
| NCBI Accession: | NP_001093882.1 |
| UniProt Related Accession: | Q9NRR4 |
| Molecular Weight: | |
| NCBI Full Name: | ribonuclease 3 isoform 2 |
| NCBI Synonym Full Names: | drosha ribonuclease III |
| NCBI Official Symbol: | DROSHA |
| NCBI Official Synonym Symbols: | RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976 |
| NCBI Protein Information: | ribonuclease 3 |
| UniProt Protein Name: | Ribonuclease 3 |
| UniProt Synonym Protein Names: | Protein Drosha |
| UniProt Gene Name: | DROSHA |
 | Western blot analysis of extracts of Mouse heart, using DROSHA Rabbit pAb (CAB8336) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of U2OS cells using DROSHA antibody (CAB8336) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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