Cell Biology Antibodies 9

Anti-RHOT1 Antibody (CAB5838)

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SKU:
CAB5838
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RHOT1 Antibody
Antibody SKU:CAB5838
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1 (NP_060777.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, U-87MG, HeLa, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1 (NP_060777.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QSTF DKHD LDRD CALS PDEL KDLF KVFP YIPW GPDV NNTV CTNE RGWI TYQG FLSQ WTLT TYLD VQRC LEYL GYLG YSIL TEQE SQAS AVTV TRDK KIDL QKKQ TQRN VFRC NVIG VKNC GKSG VLQA LLGR NLMR QKKI REDH KSYY AINT VYVY GQEK YLLL HDIS ESEF LTEA EIIC DVVC LVYD VSNP KSFE YCAR IFKQ HFMD SRIP CLIV AAKS DLHE VKQE YSIS PTDF CRKH KMPP PQAF TCNT ADAP SKDI FVKL TTMA MYPH VTQA DLKS S
Gene ID:55288
Uniprot:Q8IXI2
Cellular Location:Mitochondrion outer membrane, Single-pass type IV membrane protein
Calculated MW:28kDa/66kDa/70kDa/71kDa/74kDa/75kDa/79kDa
Observed MW:80kDa
Synonyms:RHOT1, ARHT1, MIRO-1, MIRO1
Background:
UniProt Protein Function:RHOT1: Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution. Belongs to the mitochondrial Rho GTPase family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:G protein, monomeric, Rho; Mitochondrial; G protein, monomeric; EC 3.6.5.-; Membrane protein, integral; G protein; Hydrolase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytosol; integral to mitochondrial outer membrane; membrane; plasma membrane

Molecular Function:protein binding

Biological Process: cellular homeostasis; mitochondrion transport along microtubule; regulation of small GTPase mediated signal transduction

UniProt Code:Q8IXI2
NCBI GenInfo Identifier:108860796
NCBI Gene ID:55288
NCBI Accession:Q8IXI2.2
UniProt Secondary Accession:Q8IXI2,Q6NUR3, Q6P9F8, Q6PJG1, Q6YMW8, Q86UB0, Q8IW28 Q8IXJ7, A4FVB6, A6NFV0, B4DG48, J9JIH9,
UniProt Related Accession:Q8IXI2
Molecular Weight:75,546 Da
NCBI Full Name:Mitochondrial Rho GTPase 1
NCBI Synonym Full Names:ras homolog family member T1
NCBI Official Symbol:RHOT1  
NCBI Official Synonym Symbols:ARHT1; MIRO1; MIRO-1  
NCBI Protein Information:mitochondrial Rho GTPase 1
UniProt Protein Name:Mitochondrial Rho GTPase 1
UniProt Synonym Protein Names:Rac-GTP-binding protein-like protein; Ras homolog gene family member T1
UniProt Gene Name:RHOT1  
UniProt Entry Name:MIRO1_HUMAN
Anti-RHOT1 Antibody (CAB5838)Western blot analysis of extracts of various cell lines, using RHOT1 antibody (CAB5838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RHOT1 Antibody (CAB5838)Immunohistochemistry of paraffin-embedded mouse lung using RHOT1 antibody (CAB5838) at dilution of 1:100 (40x lens).
Anti-RHOT1 Antibody (CAB5838)Immunohistochemistry of paraffin-embedded human esophagus using RHOT1 antibody (CAB5838) at dilution of 1:100 (40x lens).
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