Antibody Name:	Anti-RHOH Antibody
Antibody SKU:	CAB6356
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human RHOH (NP_004301.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	BT-474, SW620, Jurkat, HT-29, Mouse spleen, Mouse thymus, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human RHOH (NP_004301.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLSS IKCV LVGD SAVG KTSL LVRF TSET FPEA YKPT VYEN TGVD VFMD GIQI SLGL WDTA GNDA FRSI RPLS YQQA DVVL MCYS VANH NSFL NLKN KWIG EIRS NLPC TPVL VVAT QTDQ REMG PHRA SCVN AMEG KKLA QDVR AKGY LECS ALSN RGVQ QVFE CAVR TAVN QARR RNRR RLFS INEC KIF
Gene ID:	399
Uniprot:	Q15669
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor
Calculated MW:	21kDa
Observed MW:	27kDa

Synonyms:

RHOH, ARHH, TTF

Background:

The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein.

UniProt Protein Function:	RHOH: Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells. Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils. A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6. Belongs to the small GTPase superfamily. Rho family.
UniProt Protein Details:	Protein type:G protein, monomeric, Rho; G protein; G protein, monomeric Chromosomal Location of Human Ortholog: 4p13 Cellular Component: cytoplasm; plasma membrane; immunological synapse; cytosol Molecular Function:protein binding; Rho GTPase binding; GTP binding; kinase inhibitor activity; GTPase inhibitor activity Biological Process: regulation of small GTPase mediated signal transduction; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; negative regulation of catalytic activity; negative regulation of phosphorylation; negative regulation of I-kappaB kinase/NF-kappaB cascade; mast cell activation; T cell differentiation
NCBI Summary:	The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013]
UniProt Code:	Q15669
NCBI GenInfo Identifier:	2500200
NCBI Gene ID:	399
NCBI Accession:	Q15669.1
UniProt Related Accession:	Q15669
Molecular Weight:	191
NCBI Full Name:	Rho-related GTP-binding protein RhoH
NCBI Synonym Full Names:	ras homolog family member H
NCBI Official Symbol:	RHOH
NCBI Official Synonym Symbols:	TTF; ARHH
NCBI Protein Information:	rho-related GTP-binding protein RhoH; GTP-binding protein TTF; TTF, translocation three four; ras homolog gene family, member H
UniProt Protein Name:	Rho-related GTP-binding protein RhoH
UniProt Synonym Protein Names:	GTP-binding protein TTF; Translocation three four protein
Protein Family:	Rho-related GTP-binding protein
UniProt Gene Name:	RHOH
UniProt Entry Name:	RHOH_HUMAN

	Western blot analysis of extracts of various cell lines, using RHOH antibody (CAB6356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF-7 cells using RHOH antibody (CAB6356). Blue: DAPI for nuclear staining.