Antibody Name:	Anti-RHOD Antibody
Antibody SKU:	CAB6463
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human RHOD (NP_055393.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BxPC-3, Mouse kidney, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human RHOD (NP_055393.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTAA QAAG EEAP PGVR SVKV VLVG DGGC GKTS LLMV FADG AFPE SYTP TVFE RYMV NLQV KGKP VHLH IWDT AGQD DYDR LRPL FYPD ASVL LLCF DVTS PNSF DNIF NRWY PEVN HFCK KVPI IVVG CKTD LRKD KSLV NKLR RNGL EPVT YHRG QEMA RSVG AVAY LECS ARLH DNVH AVFQ EAAE VALS SRGR NFWR RITQ GFCV VT
Gene ID:	29984
Uniprot:	O00212
Cellular Location:	Cell membrane, Cytoplasmic side, Early endosome, Lipid-anchor
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:

RHOD, ARHD, RHOHP1, RHOM, Rho

Background:

Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RHOD: Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Participates in reorganization of actin cytoskeleton. Belongs to the small GTPase superfamily. Rho family.
UniProt Protein Details:	Protein type:G protein; G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11q14.3 Cellular Component: early endosome; plasma membrane; cytosol Molecular Function:GTPase activity; GTP binding; protein kinase binding Biological Process: focal adhesion formation; lamellipodium biogenesis; actin filament bundle formation; regulation of small GTPase mediated signal transduction; positive regulation of cell adhesion; metabolic process; small GTPase mediated signal transduction; regulation of focal adhesion formation; Rho protein signal transduction; protein targeting; positive regulation of cell migration
NCBI Summary:	Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:	O00212
NCBI GenInfo Identifier:	254763327
NCBI Gene ID:	29984
NCBI Accession:	O00212.2
UniProt Related Accession:	O00212
Molecular Weight:	210
NCBI Full Name:	Rho-related GTP-binding protein RhoD
NCBI Synonym Full Names:	ras homolog family member D
NCBI Official Symbol:	RHOD
NCBI Official Synonym Symbols:	Rho; ARHD; RHOM; RHOHP1
NCBI Protein Information:	rho-related GTP-binding protein RhoD; ras homolog D; Rho-related protein HP1; ras homolog gene family, member A; ras homolog gene family, member D
UniProt Protein Name:	Rho-related GTP-binding protein RhoD
UniProt Synonym Protein Names:	Rho-related protein HP1; RhoHP1
Protein Family:	Rhodopsin
UniProt Gene Name:	RHOD
UniProt Entry Name:	RHOD_HUMAN

	Western blot analysis of extracts of various cell lines, using RHOD Rabbit pAb (CAB6463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of MCF-7 cells using RHOD antibody (CAB6463). Blue: DAPI for nuclear staining.