KO Validated Antibodies 1

Anti-RHOA Antibody (CAB15641)[KO Validated]

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SKU:
CAB15641
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-RHOA Antibody [KO Validated]
Antibody SKU:CAB15641
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOA (NP_001655.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOA (NP_001655.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAI RKKL VIVG DGAC GKTC LLIV FSKD QFPE VYVP TVFE NYVA DIEV DGKQ VELA LWDT AGQE DYDR LRPL SYPD TDVI LMCF SIDS PDSL ENIP EKWT PEVK HFCP NVPI ILVG NKKD LRND EHTR RELA KMKQ EPVK PEEG RDMA NRIG AFGY MECS AKTK DGVR EVFE MATR AALQ ARRG KKKS GCLV L
Gene ID:387
Uniprot:P61586
Cellular Location:Cell membrane, Cell projection, Cleavage furrow, Cytoplasm, Cytoplasmic side, Lipid-anchor, Midbody, cell cortex, cytoskeleton, lamellipodium
Calculated MW:21kDa
Observed MW:21kDa
Synonyms:ARH12, ARHA, RHO12, RHOH12, Rho, RHOA, RhoA
Background:This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified.
UniProt Protein Function:RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.
UniProt Protein Details:

Protein type:G protein, monomeric, Rho; G protein, monomeric; Motility/polarity/chemotaxis; Oncoprotein; G protein

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: apical junction complex; focal adhesion; cytoskeleton; lamellipodium; plasma membrane; cell cortex; midbody; cytosol; cell junction; cleavage furrow

Molecular Function:GTPase activity; protein binding; myosin binding; GTP binding

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; negative chemotaxis; positive regulation of NF-kappaB import into nucleus; regulation of axonogenesis; regulation of cell migration; Rho protein signal transduction; regulation of osteoblast proliferation; substantia nigra development; transforming growth factor beta receptor signaling pathway; small GTPase mediated signal transduction; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of axonogenesis; positive regulation of cytokinesis; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; phosphoinositide-mediated signaling; apical junction assembly; regulation of small GTPase mediated signal transduction; positive regulation of axonogenesis; actin cytoskeleton organization and biogenesis; positive regulation of neuron differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation

NCBI Summary:This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
UniProt Code:P61586
NCBI GenInfo Identifier:47606458
NCBI Gene ID:387
NCBI Accession:P61586.1
UniProt Related Accession:P61586
Molecular Weight:22kDa
NCBI Full Name:Transforming protein RhoA
NCBI Synonym Full Names:ras homolog family member A
NCBI Official Symbol:RHOA  
NCBI Official Synonym Symbols:ARHA; ARH12; RHO12; RHOH12  
NCBI Protein Information:transforming protein RhoA
UniProt Protein Name:Transforming protein RhoA
UniProt Synonym Protein Names:Rho cDNA clone 12; h12
Protein Family:Rhotekin
UniProt Gene Name:RHOA  
UniProt Entry Name:RHOA_HUMAN
Anti-RHOA Antibody [KO Validated] (CAB15641)Western blot analysis of extracts from normal (control) and RHOA knockout (KO) HeLa cells, using RHOA antibody (CAB15641) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-RHOA Antibody [KO Validated] (CAB15641)Immunofluorescence analysis of U2OS cells using RHOA antibody (CAB15641) at dilution of 1:100. Blue: DAPI for nuclear staining.
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