Signal Transduction Antibodies 2

Anti-RGS4 Antibody (CAB16423)

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SKU:
CAB16423
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-RGS4 Antibody
Antibody SKU:CAB16423
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RGS4 (NP_005604.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse heart, Rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RGS4 (NP_005604.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MCKG LAGL PASC LRSA KDMK HRLG FLLQ KSDS CEHN SSHN KKDK VVIC QRVS QEEV KKWA ESLE NLIS HECG LAAF KAFL KSEY SEEN IDFW ISCE EYKK
Gene ID:5999
Uniprot:P49798
Cellular Location:
Calculated MW:10kDa/21kDa/23kDa/33kDa
Observed MW:30kDa
Synonyms:RGS4, RGP4, SCZD9
Background:Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:RGS4: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. Expressed in brain and heart. Expressed in brain at protein level. Expressed in prefontal and visual cortex. Isoform 4 and isoform 5 are expressed ubiquitously. Isoform 1, isoform 2 and isoform 3 are not expressed in the cerebellum. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; nucleus; plasma membrane

Molecular Function:calmodulin binding

Biological Process: inactivation of MAPK activity; regulation of G-protein coupled receptor protein signaling pathway

Disease: Schizophrenia 9

NCBI Summary:Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P49798
NCBI GenInfo Identifier:1710146
NCBI Gene ID:5999
NCBI Accession:P49798.1
UniProt Secondary Accession:P49798,A7XA56, A7XA58, A7XA59, A7YVV7, B1APZ3,
UniProt Related Accession:P49798
Molecular Weight:21,452 Da
NCBI Full Name:Regulator of G-protein signaling 4
NCBI Synonym Full Names:regulator of G-protein signaling 4
NCBI Official Symbol:RGS4  
NCBI Official Synonym Symbols:RGP4; SCZD9  
NCBI Protein Information:regulator of G-protein signaling 4
UniProt Protein Name:Regulator of G-protein signaling 4
Protein Family:Regulator of G-protein signaling
UniProt Gene Name:RGS4  
UniProt Entry Name:RGS4_HUMAN
Anti-RGS4 Antibody (CAB16423)Western blot analysis of extracts of various cell lines, using RGS4 antibody (CAB16423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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