Name: Anti-RGS20 Antibody (CAB8167)
Brand: Cell Biology Antibodies 11
SKU: CAB8167
Availability: OutOfStock

Antibody Name:	RGS20 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8167
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human RGS20 (NP_003693.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, NIH/3T3, SKOV3, HeLa, A-549, Mouse brain, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human RGS20 (NP_003693.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRTA DGGE PAGA SSPA GRVD GGLQ MGSE RMEM RKRQ MPAA QDTP GAAP GQPG AGSR GSNA CCFC WCCC CSCS CLTV RNQE DQRP TIAS HELR ADLP TWEE SPAP TLEE VNAW AQSF DKLM VTPA GRNA FREF LRTE FSEE NMLF WMAC EELK KEAN KNII EEKA RIIY EDYI SILS PKEV SLDS RVRE VINR NMVE PSQH IFDD AQLQ IYTL MHRD SYPR FMNS AVYK DLLQ SLSE KSIE A
Gene ID:	8601
Uniprot:	O76081
Cellular Location:	Cytoplasm, Lipid-anchor, Membrane, Nucleus
Calculated MW:	17kDa/21kDa/25kDa/27kDa/31kDa/43kDa
Observed MW:	44kDa

Synonyms:

RGS20, RGSZ1, ZGAP1, g(z)GAP, gz-GAP

Background:

The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RGS20: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G- protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GAPs, misc.; GAPs Chromosomal Location of Human Ortholog: 8q11.23 Cellular Component: cytoplasm; plasma membrane; nucleus Molecular Function:protein binding; GTPase activator activity Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
NCBI Summary:	The protein encoded by this gene belongs to the family of regulator of G protein signaling (RGS) proteins, which are regulatory and structural components of G protein-coupled receptor complexes. RGS proteins inhibit signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound forms. This protein selectively binds to G(z)-alpha and G(alpha)-i2 subunits, and regulates their signaling activities. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:	O76081
NCBI GenInfo Identifier:	18266795
NCBI Gene ID:	8601
NCBI Accession:	O76081.4
UniProt Secondary Accession:	O76081,Q96BG9,
UniProt Related Accession:	O76081
Molecular Weight:	27,060 Da
NCBI Full Name:	Regulator of G-protein signaling 20
NCBI Synonym Full Names:	regulator of G-protein signaling 20
NCBI Official Symbol:	RGS20
NCBI Official Synonym Symbols:	RGSZ1; ZGAP1; gz-GAP; g(z)GAP
NCBI Protein Information:	regulator of G-protein signaling 20; regulator of G-protein signaling Z1; regulator of G-protein signalling 20; gz-selective GTPase-activating protein; regulator of G-protein signaling 20 variant 2; regulator of Gz-selective protein signaling 1
UniProt Protein Name:	Regulator of G-protein signaling 20
UniProt Synonym Protein Names:	Gz-selective GTPase-activating protein; G(z)GAP; Gz-GAP; Regulator of G-protein signaling Z1; Regulator of Gz-selective protein signaling 1
Protein Family:	Regulator of G-protein signaling
UniProt Gene Name:	RGS20
UniProt Entry Name:	RGS20_HUMAN

Anti-RGS20 Polyclonal Antibody (CAB8167)

Western blot analysis of extracts of various cell lines, using RGS20 antibody (CAB8167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

RGS20 Antibody (PACO11821)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)