Cell Biology Antibodies 12

Anti-RGS10 Antibody (CAB9948)

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SKU:
CAB9948
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RGS10 Antibody
Antibody SKU:CAB9948
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human RGS10 (NP_001005339.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, HT-1080, Jurkat, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human RGS10 (NP_001005339.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFNR AVSR LSRK RPPS DIHD SDGS SSSS HQSL KSTA KWAA SLEN LLED PEGV KRFR EFLK KEFS EENV LFWL ACED FKKM QDKT QMQE KAKE IYMT FLSS KASS QVNV EGQS RLNE KILE EPHP LMFQ KLQD QIFN LMKY DSYS RFLK SDLF LKHK RTEE EEED LPDA QTAA KRAS RIYN T
Gene ID:6001
Uniprot:O43665
Cellular Location:
Calculated MW:19kDa/20kDa/21kDa
Observed MW:20kDa
Synonyms:RGS10
Background:Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:RGS10: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)- alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.
UniProt Protein Details:

Protein type:GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: cell soma; cytoplasm; dendritic spine; plasma membrane; nerve terminal; nucleus

Molecular Function:GTPase activator activity

Biological Process: positive regulation of GTPase activity

NCBI Summary:Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O43665
NCBI GenInfo Identifier:52694755
NCBI Gene ID:6001
NCBI Accession:NP_001005339.1
UniProt Secondary Accession:O43665,Q6IAZ6, Q96GN0, A8K408, B1AMR8,
UniProt Related Accession:O43665
Molecular Weight:20 KD
NCBI Full Name:regulator of G-protein signaling 10 isoform a
NCBI Synonym Full Names:regulator of G-protein signaling 10
NCBI Official Symbol:RGS10  
NCBI Protein Information:regulator of G-protein signaling 10; regulator of G-protein signalling 10
UniProt Protein Name:Regulator of G-protein signaling 10
Protein Family:Regulator of G-protein signaling
UniProt Gene Name:RGS10  
UniProt Entry Name:RGS10_HUMAN
Anti-RGS10 Polyclonal Antibody (CAB9948)Western blot analysis of extracts of various cell lines, using RGS10 antibody (CAB9948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
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