Epigenetics & Nuclear Signaling Antibodies 2

Anti-RFX1 Antibody (CAB16066)

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SKU:
CAB16066
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RFX1 Antibody
Antibody SKU:CAB16066
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RFX1 (NP_002909.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human RFX1 (NP_002909.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QQVH SPPE QSPV QANS SSSK TAGA PTGT VPQQ LQVH GVQQ SVPV TQER SVVQ ATPQ APKP GPVQ PLTV QGLQ PVHV AQEV QQLQ QVPV PHVY SSQV QYVE G
Gene ID:5989
Uniprot:P22670
Cellular Location:Nucleus
Calculated MW:104kDa
Observed MW:130kDa
Synonyms:RFX1, EFC, RFX
Background:This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE).
UniProt Protein Function:RFX1: Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter. Belongs to the RFX family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function:protein binding; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: immune response

NCBI Summary:This gene encodes a member of the regulatory factor X (RFX) family of transcription factors, which are characterized by a winged-helix DNA-binding domain. The encoded transcription factor contains an N-terminal activation domain and a C-terminal repression domain, and may activate or repress target gene expression depending on cellular context. This transcription factor has been shown to regulate a wide variety of genes involved in immunity and cancer, including the MHC class II genes and genes that may be involved in cancer progression. This gene exhibits altered expression in glioblastoma and the autoimmune disease systemic lupus erythematosis (SLE). [provided by RefSeq, Jul 2016]
UniProt Code:P22670
NCBI GenInfo Identifier:288558824
NCBI Gene ID:5989
NCBI Accession:P22670.2
UniProt Related Accession:P22670
Molecular Weight:104,758 Da
NCBI Full Name:MHC class II regulatory factor RFX1
NCBI Synonym Full Names:regulatory factor X1
NCBI Official Symbol:RFX1  
NCBI Official Synonym Symbols:EFC; RFX  
NCBI Protein Information:MHC class II regulatory factor RFX1
UniProt Protein Name:MHC class II regulatory factor RFX1
UniProt Synonym Protein Names:Enhancer factor C; EF-C; Regulatory factor X 1; RFX; Transcription factor RFX1
Protein Family:RFX-like DNA-binding protein
UniProt Gene Name:RFX1  
UniProt Entry Name:RFX1_HUMAN
Anti-RFX1 Antibody (CAB16066)Western blot analysis of extracts of LO2 cells, using RFX1 antibody (CAB16066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
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