Signal Transduction Antibodies 1

Anti-RFWD2 Antibody (CAB10463)

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SKU:
CAB10463
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-RFWD2 Antibody
Antibody SKU:CAB10463
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human RFWD2 (NP_071902.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 582-731 of human RFWD2 (NP_071902.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YYDL RNTK QPIM VFKG HRKA VSYA KFVS GEEI VSAS TDSQ LKLW NVGK PYCL RSFK GHIN EKNF VGLA SNGD YIAC GSEN NSLY LYYK GLSK TLLT FKFD TVKS VLDK DRKE DDTN EFVS AVCW RALP DGES NVLI AANS QGTI KVLE LV
Gene ID:64326
Uniprot:Q8NHY2
Cellular Location:Cytoplasm, Nucleus speckle
Calculated MW:14kDa/57kDa/77kDa/78kDa/80kDa
Observed MW:110kDa
Synonyms:RFWD2, COP1, RNF200
Background:
UniProt Protein Function:COP1: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Homodimer. Homodimerization is mediated by the coiled coil domain. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Isoform 2 does not interact with CUL4A but still binds to RBX1, suggesting that the interaction may be mediated by another culllin protein. Isoform 1 and isoform 2 interact with CUL5 but not with CUL1, CUL2 not CUL3. Interacts with bZIP transcription factors JUN, JUNB and JUND but not with FOS, ATF2 nor XBP1. Interacts with p53 (TP53). By p53/TP53. Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. Belongs to the COP1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 1q25.1-q25.2

Cellular Component: centrosome; cytoplasm; cytosol; focal adhesion; Golgi membrane; nuclear speck; nucleoplasm

Molecular Function:ligase activity; protein binding; zinc ion binding

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; response to ionizing radiation

UniProt Code:Q8NHY2
NCBI GenInfo Identifier:55976539
NCBI Gene ID:64326
NCBI Accession:Q8NHY2.1
UniProt Secondary Accession:Q8NHY2,Q504W6, Q6H103, Q9H6L7, E9PKI0, X5D9B4,
UniProt Related Accession:Q8NHY2
Molecular Weight:14,914 Da
NCBI Full Name:E3 ubiquitin-protein ligase RFWD2
NCBI Synonym Full Names:ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
NCBI Official Symbol:RFWD2  
NCBI Official Synonym Symbols:COP1; RNF200  
NCBI Protein Information:E3 ubiquitin-protein ligase RFWD2
UniProt Protein Name:E3 ubiquitin-protein ligase RFWD2
UniProt Synonym Protein Names:Constitutive photomorphogenesis protein 1 homolog; hCOP1; RING finger and WD repeat domain protein 2; RING finger protein 200
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RFWD2  
UniProt Entry Name:RFWD2_HUMAN
Anti-RFWD2 Antibody (CAB10463)Western blot analysis of extracts of various cell lines, using RFWD2 antibody (CAB10463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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