Antibody Name:	RFC5 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8056
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 181-340 of human RFC5 (NP_031396.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, U-251MG, SKOV3, MCF-7, Mouse liver, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 181-340 of human RFC5 (NP_031396.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LMVP RLEH VVEE EKVD ISED GMKA LVTL SSGD MRRA LNIL QSTN MAFG KVTE ETVY TCTG HPLK SDIA NILD WMLN QDFT TAYR NITE LKTL KGLA LHDI LTEI HLFV HRVD FPSS VRIH LLTK MADI EYRL SVGT NEKI QLSS LIAA FQVT RDLI VAEA
Gene ID:	5985
Uniprot:	P40937
Cellular Location:	Nucleus
Calculated MW:	36kDa/38kDa
Observed MW:	38kDa

Synonyms:

RFC5, RFC36

Background:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.

UniProt Protein Function:	RFC5: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Belongs to the activator 1 small subunits family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 12q24.23 Cellular Component: DNA replication factor C complex; nucleoplasm Molecular Function:DNA clamp loader activity; protein binding; single-stranded DNA-dependent ATPase activity Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA replication; error-prone postreplication DNA repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair
NCBI Summary:	The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9. [provided by RefSeq, May 2011]
UniProt Code:	P40937
NCBI GenInfo Identifier:	728777
NCBI Gene ID:	5985
NCBI Accession:	P40937.1
UniProt Secondary Accession:	P40937,A8MZ62, B3KSX8,
UniProt Related Accession:	P40937
Molecular Weight:	36,105 Da
NCBI Full Name:	Replication factor C subunit 5
NCBI Synonym Full Names:	replication factor C subunit 5
NCBI Official Symbol:	RFC5
NCBI Official Synonym Symbols:	RFC36
NCBI Protein Information:	replication factor C subunit 5
UniProt Protein Name:	Replication factor C subunit 5
UniProt Synonym Protein Names:	Activator 1 36 kDa subunit; A1 36 kDa subunit; Activator 1 subunit 5; Replication factor C 36 kDa subunit; RF-C 36 kDa subunit; RFC36
Protein Family:	Replication factor
UniProt Gene Name:	RFC5
UniProt Entry Name:	RFC5_HUMAN

	Western blot analysis of extracts of various cell lines, using RFC5 antibody (CAB8056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human lung cancer using RFC5 antibody (CAB8056) at dilution of 1:100 (40x lens).