Name: Anti-RFC3 Antibody (CAB4075)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB4075
Availability: OutOfStock

Antibody Name:	Anti-RFC3 Antibody
Antibody SKU:	CAB4075
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RFC3 (NP_002906.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, SW480, HL-60, NIH/3T3, 293T, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RFC3 (NP_002906.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLW VDKY RPCS LGRL DYHK EQAA QLRN LVQC GDFP HLLV YGPS GAGK KTRI MCIL RELY GVGV EKLR IEHQ TITT PSKK KIEI STIA SNYH LEVN PSDA GNSD RVVI QEML KTVA QSQQ LETN SQRD FKVV LLTE VDKL TKDA QHAL RRTM EKYM STCR LILC CNST SKVI PPIR SRCL AVRV PAPS IEDI CHVL STVC KKEG LNLP SQLA HRLA EKSC RNLR KALL MCEA CRVQ QYPF TADQ EIPE TDWE VYLR ETAN AIVS QQTP QRLL EVRG RLYE
Gene ID:	5983
Uniprot:	P40938
Cellular Location:	Nucleus
Calculated MW:	34kDa/40kDa
Observed MW:	41kDa

Synonyms:

RFC3, RFC38

Background:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	RFC3: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Belongs to the activator 1 small subunits family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 13q13.2 Cellular Component: nucleoplasm; DNA replication factor C complex; nucleus Molecular Function:protein binding; DNA binding; ATPase activity; DNA clamp loader activity Biological Process: telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; response to organophosphorus; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; DNA repair; DNA replication; telomere maintenance
NCBI Summary:	The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P40938
NCBI GenInfo Identifier:	3915601
NCBI Gene ID:	5983
NCBI Accession:	P40938.2
UniProt Secondary Accession:	P40938,O15252, Q5W0E8, C9JU95,
UniProt Related Accession:	P40938
Molecular Weight:	356
NCBI Full Name:	Replication factor C subunit 3
NCBI Synonym Full Names:	replication factor C (activator 1) 3, 38kDa
NCBI Official Symbol:	RFC3
NCBI Official Synonym Symbols:	RFC38
NCBI Protein Information:	replication factor C subunit 3; A1 38 kDa subunit; RFC, 38 kD subunit; RF-C 38 kDa subunit; activator 1 subunit 3; activator 1 38 kDa subunit; replication factor C 38 kDa subunit
UniProt Protein Name:	Replication factor C subunit 3
UniProt Synonym Protein Names:	Activator 1 38 kDa subunit; A1 38 kDa subunit; Activator 1 subunit 3; Replication factor C 38 kDa subunit; RF-C 38 kDa subunit; RFC38
Protein Family:	Replication factor
UniProt Gene Name:	RFC3
UniProt Entry Name:	RFC3_HUMAN

Western blot analysis of extracts of various cell lines, using RFC3 antibody (CAB4075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

RFC3 Antibody (PACO01422)

RFC3 Antibody (PACO11805)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)