Antibody Name:	Anti-RFC1 Antibody
Antibody SKU:	CAB1625
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human RFC1 (NP_001191676.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PKGA ENCL EGLI FVIT GVLE SIER DEAK SLIE RYGG KVTG NVSK KTNY LVMG RDSG QSKS DKAA ALGT KIID EDGL LNLI RTMP GKKS KYEI AVET EMKK ESKL ERTP QKNV QGKR KISP SKKE SESK KSRP TSKR DSLA KTIK KETD VFWK SLDF KEQV AEET SGDS KARN LADD SSEN KVEN LLWV DKYK PTSL KTII GQQG DQSC ANKL LRWL RNWQ KSSS EDKK HAAK FGKF SGKD DGSS FKAA LLSG PPGV GKTT TASL VCQE LGYS YVEL NASD TRSK SSLK AIVA ESLN NTSI K
Gene ID:	5981
Uniprot:	P35251
Cellular Location:	Nucleus
Calculated MW:	128kDa
Observed MW:	130kDa

Synonyms:

RFC1, A1, MHCBFB, PO-GA, RECC1, RFC, RFC140

Background:

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt Protein Function:	RFC1: a DNA binding protein that may play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and RFC1. The RFC1 subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:DNA replication Chromosomal Location of Human Ortholog: 4p14-p13 Cellular Component: nucleoplasm; Golgi apparatus; DNA replication factor C complex; nucleolus; cell junction; nucleus Molecular Function:protein domain specific binding; protein binding; DNA binding; sequence-specific DNA binding; enzyme activator activity; double-stranded DNA binding; ATP binding; DNA clamp loader activity Biological Process: positive regulation of catalytic activity; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; telomere maintenance via telomerase; negative regulation of transcription from RNA polymerase II promoter; DNA strand elongation during DNA replication; DNA repair; telomere maintenance via semi-conservative replication; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA-dependent DNA replication; telomere maintenance
NCBI Summary:	This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]
UniProt Code:	P35251
NCBI GenInfo Identifier:	56757608
NCBI Gene ID:	5981
NCBI Accession:	P35251.4
UniProt Secondary Accession:	P35251,Q5XKF5, Q6PKU0, Q86V41, Q86V46, A8K6E7,
UniProt Related Accession:	P35251
Molecular Weight:	1148
NCBI Full Name:	Replication factor C subunit 1
NCBI Synonym Full Names:	replication factor C (activator 1) 1, 145kDa
NCBI Official Symbol:	RFC1
NCBI Official Synonym Symbols:	A1; RFC; PO-GA; RECC1; MHCBFB; RFC140
NCBI Protein Information:	replication factor C subunit 1; A1 140 kDa subunit; RF-C 140 kDa subunit; activator 1 subunit 1; replication factor C1; MHC binding factor, beta; DNA-binding protein PO-GA; activator 1 large subunit; activator 1 140 kDa subunit; replication factor C large
UniProt Protein Name:	Replication factor C subunit 1
UniProt Synonym Protein Names:	Activator 1 140 kDa subunit; A1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GA; Replication factor C 140 kDa subunit; RF-C 140 kDa subunit; RFC140; Replication factor C large subunit
Protein Family:	O-antigen polymerase
UniProt Gene Name:	RFC1
UniProt Entry Name:	RFC1_HUMAN

	Western blot analysis of extracts of MCF-7 cells, using RFC1 antibody (CAB1625) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of MCF-7 cells using RFC1 antibody (CAB1625). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of A549 cells using RFC1 antibody (CAB1625).