Cell Biology Antibodies 6
Anti-REXO2 Antibody (CAB15808)
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- SKU:
- CAB15808
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-REXO2 Antibody |
| Antibody SKU: | CAB15808 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-237 of human REXO2 (NP_056338.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, U-87MG, LO2, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-237 of human REXO2 (NP_056338.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VREG GAAM AAGE SMAQ RMVW VDLE MTGL DIEK DQII EMAC LITD SDLN ILAE GPNL IIKQ PDEL LDSM SDWC KEHH GKSG LTKA VKES TITL QQAE YEFL SFVR QQTP PGLC PLAG NSVH EDKK FLDK YMPQ FMKH LHYR IIDV STVK ELCR RWYP EEYE FAPK KAAS HRAL DDIS ESIK ELQF YRNN IFKK KIDE KKRK IIEN GENE KTVS |
| Gene ID: | 25996 |
| Uniprot: | Q9Y3B8 |
| Cellular Location: | Cytoplasm, Mitochondrion intermembrane space, Mitochondrion matrix, Nucleus |
| Calculated MW: | 23kDa/26kDa |
| Observed MW: | 27kDa |
| Synonyms: | REXO2, CGI-114, REX2, RFN, SFN |
| Background: | This gene encodes a 3'-to-5' exonuclease specific for small (primarily 5 nucleotides or less in length) single-stranded RNA and DNA oligomers. This protein may have a role in DNA repair, replication, and recombination, and in RNA processing and degradation. It may also be involved in resistance of human cells to UV-C-induced cell death through its role in the DNA repair process. |
| UniProt Protein Function: | REXO2: a 3'-to-5' exoribonuclease specific for small oligoribonucleotides. Active on small (primarily |
| UniProt Protein Details: | Protein type:EC 3.1.-.-; Hydrolase Chromosomal Location of Human Ortholog: 11q23.2 Cellular Component: focal adhesion; mitochondrion; nucleolus; nucleus Molecular Function:3'-5' exonuclease activity; 3'-5'-exoribonuclease activity Biological Process: nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleotide metabolic process |
| NCBI Summary: | This gene encodes a 3'-to-5' exonuclease specific for small (primarily 5 nucleotides or less in length) single-stranded RNA and DNA oligomers. This protein may have a role in DNA repair, replication, and recombination, and in RNA processing and degradation. It may also be involved in resistance of human cells to UV-C-induced cell death through its role in the DNA repair process. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q9Y3B8 |
| NCBI GenInfo Identifier: | 116242694 |
| NCBI Gene ID: | 25996 |
| NCBI Accession: | Q9Y3B8.3 |
| UniProt Secondary Accession: | Q9Y3B8,Q32Q18, Q53FT1, Q6FIC6, Q9UFY7, B2R532, |
| UniProt Related Accession: | Q9Y3B8 |
| Molecular Weight: | 23,754 Da |
| NCBI Full Name: | Oligoribonuclease, mitochondrial |
| NCBI Synonym Full Names: | RNA exonuclease 2 |
| NCBI Official Symbol: | REXO2 |
| NCBI Official Synonym Symbols: | RFN; SFN; REX2; CGI-114 |
| NCBI Protein Information: | oligoribonuclease, mitochondrial |
| UniProt Protein Name: | Oligoribonuclease, mitochondrial |
| UniProt Synonym Protein Names: | RNA exonuclease 2 homolog; Small fragment nuclease |
| UniProt Gene Name: | REXO2 |
| UniProt Entry Name: | ORN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using REXO2 antibody (CAB15808) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of L929 cells using REXO2 Polyclonal Antibody (CAB15808) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using REXO2 Polyclonal Antibody (CAB15808) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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