Epigenetics & Nuclear Signaling Antibodies 4

Anti-REV1 Antibody (CAB8493)

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SKU:
CAB8493
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-REV1 Antibody
Antibody SKU:CAB8493
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-345 of human REV1 (NP_057400.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-345 of human REV1 (NP_057400.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AGRL LSYI PYQL YTKQ SSVQ KGLS FNPV CRPE DPLP GPSN IAKQ LNNR VNHI VKKI ETEN EVKV NGMN SWNE EDEN NDFS FVDL EQTS PGRK QNGI PHPR GSTA IFNG HTPS SNGA LKTQ DCLV PMVN SVAS RLSP AFSQ EEDK AEKS STDF RDCT LQQL QQST RNTD ALRN PHRT NSFS LSPL HSNT KING AHHS TVQG PSST KSTS SVST FSKA APSV PSKP SD
Gene ID:51455
Uniprot:Q9UBZ9
Cellular Location:Nucleus
Calculated MW:43kDa/138kDa
Observed MW:145kDa
Synonyms:REV1, AIBP80, REV1L
Background:This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA.
UniProt Protein Function:Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
NCBI Summary:This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
UniProt Code:Q9UBZ9
NCBI GenInfo Identifier:84043967
NCBI Gene ID:51455
NCBI Accession:NP_001032961.1
UniProt Secondary Accession:Q9UBZ9,O95941, Q53SI7, Q9C0J4, Q9NUP2,
UniProt Related Accession:Q9UBZ9
Molecular Weight:Observed: 138 kDaPredicted: 139 kDa
NCBI Full Name:DNA repair protein REV1 isoform 2
NCBI Synonym Full Names:REV1, DNA directed polymerase
NCBI Official Symbol:REV1  
NCBI Official Synonym Symbols:REV1L; AIBP80  
NCBI Protein Information:DNA repair protein REV1
UniProt Protein Name:DNA repair protein REV1
UniProt Synonym Protein Names:Alpha integrin-binding protein 80; AIBP80; Rev1-like terminal deoxycytidyl transferase
Protein Family:DNA repair protein
UniProt Gene Name:REV1  
Anti-REV1 Polyclonal Antibody (CAB8493)Western blot analysis of extracts of MCF-7 cells, using REV1 antibody (CAB8493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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