Name: Anti-REEP1 Antibody (CAB7832)
Brand: Cell Biology Antibodies 11
SKU: CAB7832
Availability: OutOfStock

Antibody Name:	Anti-REEP1 Antibody
Antibody SKU:	CAB7832
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-201 of human REEP1 (NP_075063.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-201 of human REEP1 (NP_075063.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KEID DCLV QAKD RSYD ALVH FGKR GLNV AATA AVMA ASKG QGAL SERL RSFS MQDL TTIR GDGA PAPS GPPP PGSG RASG KHGQ PKMS RSAS ESAS SSGT A
Gene ID:	65055
Uniprot:	Q9H902
Cellular Location:	Endoplasmic reticulum, Membrane, Mitochondrion membrane, Multi-pass membrane protein
Calculated MW:	16kDa/18kDa/22kDa
Observed MW:	22kDa

Synonyms:	REEP1, C2orf23, HMN5B, SPG31, Yip2a
Background:	This gene encodes a mitochondrial protein that functions to enhance the cell surface expression of odorant receptors. Mutations in this gene cause spastic paraplegia autosomal dominant type 31, a neurodegenerative disorder. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	REEP1: Required for endoplasmic reticulum (ER) network formation, shaping and remodeling; it links ER tubules to the cytoskeleton. May also enhance the cell surface expression of odorant receptors. Defects in REEP1 are the cause of spastic paraplegia autosomal dominant type 31 (SPG31). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the DP1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; mitochondrial membrane; integral to membrane Molecular Function:olfactory receptor binding; protein binding; microtubule binding Biological Process: regulation of intracellular transport; protein insertion into membrane Disease: Neuronopathy, Distal Hereditary Motor, Type Vb; Spastic Paraplegia 31, Autosomal Dominant
NCBI Summary:	This gene encodes a mitochondrial protein that functions to enhance the cell surface expression of odorant receptors. Mutations in this gene cause spastic paraplegia autosomal dominant type 31, a neurodegenerative disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Code:	Q9H902
NCBI GenInfo Identifier:	257900516
NCBI Gene ID:	65055
NCBI Accession:	NP_001158202.1
UniProt Related Accession:	Q9H902
Molecular Weight:	30kDa
NCBI Full Name:	receptor expression-enhancing protein 1 isoform 1
NCBI Synonym Full Names:	receptor accessory protein 1
NCBI Official Symbol:	REEP1
NCBI Official Synonym Symbols:	HMN5B; SPG31; Yip2a; C2orf23
NCBI Protein Information:	receptor expression-enhancing protein 1
UniProt Protein Name:	Receptor expression-enhancing protein 1
Protein Family:	Receptor expression-enhancing protein
UniProt Gene Name:	REEP1
UniProt Entry Name:	REEP1_HUMAN

	Western blot analysis of extracts of various cell lines, using REEP1 antibody (CAB7832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using REEP1 antibody (CAB7832) at dilution of 1:100. Blue: DAPI for nuclear staining.