Cell Biology Antibodies 10

Anti-RECQL4 Antibody (CAB6846)

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SKU:
CAB6846
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RECQL4 Antibody
Antibody SKU:CAB6846
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 231-330 of human RECQL4 (NP_004251.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 231-330 of human RECQL4 (NP_004251.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GAGS QGPE ASAF QEVS IRVG SPQP SSSG GEKR RWNE EPWE SPAQ VQQE SSQA GPPS EGAG AVAV EEDP PGEP VQAQ PPQP CSSP SNPR YHGL SPSS QARA
Gene ID:9401
Uniprot:O94761
Cellular Location:Cytoplasm, Nucleus
Calculated MW:133kDa
Observed MW:140kDa
Synonyms:RECQL4, RECQ4
Background:The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes.
UniProt Protein Function:RECQL4: DNA-dependent ATPase. May modulate chromosome segregation. Interacts with UBR1 and UBR2. Interacts with MCM10; this interaction regulates RECQL4 unwinding activity. Up-regulated in actively dividing cells. Ubiquitously expressed, with highest levels in thymus and testis. Belongs to the helicase family. RecQ subfamily.
UniProt Protein Details:

Protein type:Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: membrane; cytoplasm; nucleus

Molecular Function:ATP-dependent 3'-5' DNA helicase activity; zinc ion binding; bubble DNA binding; ATP binding

Biological Process: multicellular organismal development; DNA strand renaturation; DNA replication; DNA repair; DNA duplex unwinding; DNA recombination

Disease: Rapadilino Syndrome; Rothmund-thomson Syndrome; Baller-gerold Syndrome

NCBI Summary:The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010]
UniProt Code:O94761
NCBI GenInfo Identifier:18206225
NCBI Gene ID:9401
NCBI Accession:O94761.1
UniProt Secondary Accession:O94761,Q3Y424, Q96DW2, Q96F55,
UniProt Related Accession:O94761
Molecular Weight:1208
NCBI Full Name:ATP-dependent DNA helicase Q4
NCBI Synonym Full Names:RecQ protein-like 4
NCBI Official Symbol:RECQL4  
NCBI Official Synonym Symbols:RECQ4  
NCBI Protein Information:ATP-dependent DNA helicase Q4; DNA helicase, RecQ-like type 4; DNA helicase, RecQ-like, type 4
UniProt Protein Name:ATP-dependent DNA helicase Q4
UniProt Synonym Protein Names:DNA helicase, RecQ-like type 4; RecQ4; RTS; RecQ protein-like 4
Protein Family:ATP-dependent DNA helicase
UniProt Gene Name:RECQL4  
UniProt Entry Name:RECQ4_HUMAN
Anti-RECQL4 Antibody (CAB6846)Western blot analysis of extracts of various cell lines, using RECQL4 antibody (CAB6846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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