Antibody Name:	RDH5 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8055
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, BT-474, Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LPAS NAFV FITG CDSG FGRL LALQ LDQR GFRV LASC LTPS GAED LQRV ASSR LHTT LLDI TDPQ SVQQ AAKW VEMH VKEA GLFG LVNN AGVA GIIG PTPW LTRD DFQR VLNV NTMG PIGV TLAL LPLL QQAR GRVI NITS VLGR LAAN GGGY CVSK FGLE AFSD SLRR DVAH FGIR VSIV EPGF FRTP VTNL ESLE KTLQ ACWA RLPP ATQA HYGG AFLT KYLK MQQR IMNL ICDP DLTK VSRC LEHA LTAR HPRT RYSP GWDA KLLW LPAS YLPA SLVD AVLT WVLP KPAQ AVY
Gene ID:	5959
Uniprot:	Q92781
Cellular Location:	Endoplasmic reticulum lumen, Membrane, Peripheral membrane protein
Calculated MW:	34kDa
Observed MW:	38kDa

Synonyms:

RDH5, 9cRDH, HSD17B9, RDH1, SDR9C5

Background:

This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S1 (biogenesis of lysosomal organelles complex-1, subunit 1) gene.

UniProt Protein Function:	RDH5: Stereospecific 11-cis retinol dehydrogenase, which catalyzes the final step in the biosynthesis of 11-cis retinaldehyde, the universal chromophore of visual pigments. Also able to oxidize 9-cis-retinol and 13-cis-retinol, but not all- trans-retinol. Active in the presence of NAD as cofactor but not in the presence of NADP. Defects in RDH5 are a cause of retinitis punctata albescens (RPA); also known as fundus albipunctatus (FA). RPA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:	Protein type:Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; Oxidoreductase; EC 1.1.1.315 Chromosomal Location of Human Ortholog: 12q13-q14 Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum lumen Molecular Function:retinol dehydrogenase activity Biological Process: phototransduction, visible light; visual perception; retinol metabolic process; retinoid metabolic process Disease: Fundus Albipunctatus
NCBI Summary:	This gene encodes an enzyme belonging to the short-chain dehydrogenases/reductases (SDR) family. This retinol dehydrogenase functions to catalyze the final step in the biosynthesis of 11-cis retinaldehyde, which is the universal chromophore of visual pigments. Mutations in this gene cause autosomal recessive fundus albipunctatus, a rare form of night blindness that is characterized by a delay in the regeneration of cone and rod photopigments. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S1 (biogenesis of lysosomal organelles complex-1, subunit 1) gene. [provided by RefSeq, Dec 2010]
UniProt Code:	Q92781
NCBI GenInfo Identifier:	2492753
NCBI Gene ID:	5959
NCBI Accession:	Q92781.1
UniProt Secondary Accession:	Q92781,O00179, Q8TAI2,
UniProt Related Accession:	Q92781
Molecular Weight:	34,979 Da
NCBI Full Name:	11-cis retinol dehydrogenase
NCBI Synonym Full Names:	retinol dehydrogenase 5 (11-cis/9-cis)
NCBI Official Symbol:	RDH5
NCBI Official Synonym Symbols:	RDH1; 9cRDH; SDR9C5; HSD17B9
NCBI Protein Information:	11-cis retinol dehydrogenase; 11-cis RDH; 11-cis RoDH; retinol dehydrogenase 1; 9-cis retinol dehydrogenase; 9-cis-retinol specific dehydrogenase; retinol dehydrogenase 5 (11-cis and 9-cis); short chain dehydrogenase/reductase family 9C, member 5
UniProt Protein Name:	11-cis retinol dehydrogenase
UniProt Synonym Protein Names:	9-cis retinol dehydrogenase; 9cRDH
Protein Family:	11-cis retinol dehydrogenase
UniProt Gene Name:	RDH5
UniProt Entry Name:	RDH1_HUMAN

	Western blot analysis of extracts of various cell lines, using RDH5 antibody (CAB8055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of rat retina using Anti-RDH5 Polyclonal Antibody (CAB8055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse retina using Anti-RDH5 Polyclonal Antibody (CAB8055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.