Cell Biology Antibodies 2

Anti-RCN1 Antibody (CAB10572)

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SKU:
CAB10572
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RCN1 Antibody
Antibody SKU:CAB10572
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-331 of human RCN1 (NP_002892.1).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-549, SKOV3, LO2, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-331 of human RCN1 (NP_002892.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KPTV RKER VVRP DSEL GERP PEDN QSFQ YDHE AFLG KEDS KTFD QLTP DESK ERLG KIVD RIDN DGDG FVTT EELK TWIK RVQK RYIF DNVA KVWK DYDR DKDD KISW EEYK QATY GYYL GNPA EFHD SSDH HTFK KMLP RDER RFKA ADLN GDLT ATRE EFTA FLHP EEFE HMKE IVVL ETLE DIDK NGDG FVDQ DEYI ADMF SHEE NGPE PDWV LSER EQFN EFRD LNKD GKLD KDEI RHWI LPQD YDHA QAEA RHLV YESD KNKD EKLT KEEI LENW NMFV GSQA TNYG EDLT KNHD EL
Gene ID:5954
Uniprot:Q15293
Cellular Location:Endoplasmic reticulum lumen
Calculated MW:33kDa/38kDa
Observed MW:45kDa
Synonyms:RCN1, HEL-S-84, PIG20, RCAL, RCN
Background:Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.
UniProt Protein Function:RCN1: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family.
UniProt Protein Details:

Protein type:Calcium-binding

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: endoplasmic reticulum

Molecular Function:protein binding

NCBI Summary:Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]
UniProt Code:Q15293
NCBI GenInfo Identifier:2493462
NCBI Gene ID:5954
NCBI Accession:Q15293.1
UniProt Secondary Accession:Q15293,B7Z1M1, D3DR00,
UniProt Related Accession:Q15293
Molecular Weight:39kDa
NCBI Full Name:Reticulocalbin-1
NCBI Synonym Full Names:reticulocalbin 1
NCBI Official Symbol:RCN1  
NCBI Official Synonym Symbols:RCN; RCAL; PIG20; HEL-S-84  
NCBI Protein Information:reticulocalbin-1
UniProt Protein Name:Reticulocalbin-1
Protein Family:Reticulocalbin
UniProt Gene Name:RCN1  
Anti-RCN1 Antibody (CAB10572)Western blot analysis of extracts of various cell lines, using RCN1 antibody (CAB10572) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RCN1 Antibody (CAB10572)Immunofluorescence analysis of L929 cells using RCN1 Rabbit pAb (CAB10572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-RCN1 Antibody (CAB10572)Immunofluorescence analysis of U-2 OS cells using RCN1 Rabbit pAb (CAB10572) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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