Signal Transduction Antibodies 2

Anti-RCHY1 Antibody (CAB2789)

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SKU:
CAB2789
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-RCHY1 Antibody
Antibody SKU:CAB2789
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human RCHY1 (NP_056251.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:1000
Reactivity:Human, Mouse
Positive Samples:SH-SY5Y, HeLa, BT-474, SW620, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human RCHY1 (NP_056251.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAT ARED GASG QERG QRGC EHYD RGCL LKAP CCDK LYTC RLCH DNNE DHQL DRFK VKEV QCIN CEKI QHAQ QTCE ECST LFGE YYCD ICHL FDKD KKQY HCEN CGIC RIGP KEDF FHCL KCNL CLAM NLQG RHKC IENV SRQN CPIC LEDI HTSR VVAH VLPC GHLL HRTC YEEM LKEG YRCP LCMH SALD MTRY WRQL DDEV AQTP MPSE YQNM TVDI LCND CNGR STVQ FHIL GMKC KICE SYNT AQAG GRRI SLDQ Q
Gene ID:25898
Uniprot:Q96PM5
Cellular Location:Cytoplasm, Nucleus, Nucleus speckle
Calculated MW:8kDa/20-30kDa
Observed MW:30kDa
Synonyms:RCHY1, ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363
Background:The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:RCHY1: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and p27Kip1. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of p27Kip1 and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, p27Kip1, COPE, UBE2D2 and GORAB/NTKLBP1. Up-regulated during the S phase of the cell cycle. Expressed at low levels during G phase. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ubiquitin conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus; ubiquitin ligase complex

Molecular Function:protein binding; protein homodimerization activity; zinc ion binding; p53 binding; ubiquitin-protein ligase activity; ligase activity; receptor binding

Biological Process: protein autoubiquitination; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination

NCBI Summary:The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013]
UniProt Code:Q96PM5
NCBI GenInfo Identifier:32700008
NCBI Gene ID:25898
NCBI Accession:Q96PM5.1
UniProt Related Accession:Q96PM5
Molecular Weight:30 kDa
NCBI Full Name:RING finger and CHY zinc finger domain-containing protein 1
NCBI Synonym Full Names:ring finger and CHY zinc finger domain containing 1
NCBI Official Symbol:RCHY1  
NCBI Official Synonym Symbols:ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996  
NCBI Protein Information:RING finger and CHY zinc finger domain-containing protein 1
UniProt Protein Name:RING finger and CHY zinc finger domain-containing protein 1
UniProt Synonym Protein Names:Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; Zinc finger protein 363; p53-induced RING-H2 protein; hPirh2
Protein Family:RING finger and CHY zinc finger domain-containing protein
UniProt Gene Name:RCHY1  
UniProt Entry Name:ZN363_HUMAN
Anti-RCHY1 Antibody (CAB2789)Western blot analysis of extracts of various cell lines, using RCHY1 antibody (CAB2789) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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OverviewProduct Name:RCHY1 Rabbit mAbProduct Code:CAB19287Size:20uL, 50uL, 100uLSynonyms:RCHY1, ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZC