Cell Cycle Antibodies 1
Anti-RCC1 Antibody (CAB6350)
- SKU:
- CAB6350
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-RCC1 Antibody |
| Antibody SKU: | CAB6350 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RCC1 (NP_001260.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, MCF7, HeLa, SW620, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RCC1 (NP_001260.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSPK RIAK RRSP PADA IPKS KKVK VSHR SHST EPGL VLTL GQGD VGQL GLGE NVME RKKP ALVS IPED VVQA EAGG MHTV CLSK SGQV YSFG CNDE GALG RDTS VEGS EMVP GKVE LQEK VVQV SAGD SHTA ALTD DGRV FLWG SFRD NNGV IGLL EPMK KSMV PVQV QLDV PVVK VASG NDHL VMLT ADGD LYTL GCGE QGQL GRVP ELFA NRGG RQGL ERLL VPKC VMLK SRGS RGHV |
| Gene ID: | 1104 |
| Uniprot: | P18754 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 44kDa/48kDa |
| Observed MW: | 45kDa |
| Synonyms: | RCC1, CHC1, RCC1-I, SNHG3-RCC1 |
| Background: |
| UniProt Protein Function: | RCC1: a guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP for GTP. Localised generation of Ran-GTP by RCC1 on chromatin is critical for nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo- cytoplasmic transport, mitosis and nuclear-envelope assembly. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:GEFs; GEFs, Ras; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: nucleoplasm; nuclear membrane; condensed nuclear chromosome; cytoplasm; nuclear chromatin; nucleus Molecular Function:protein binding; nucleosomal DNA binding; Ran guanyl-nucleotide exchange factor activity; histone binding; chromatin binding Biological Process: mitosis; mitotic spindle organization and biogenesis; viral reproduction; cell division; regulation of mitosis; spindle assembly; G1/S transition of mitotic cell cycle; chromosome segregation |
| UniProt Code: | P18754 |
| NCBI GenInfo Identifier: | 132170 |
| NCBI Gene ID: | 1104 |
| NCBI Accession: | P18754.1 |
| UniProt Secondary Accession: | P18754,Q16269, Q6NT97, |
| UniProt Related Accession: | P18754 |
| Molecular Weight: | 421 |
| NCBI Full Name: | Regulator of chromosome condensation |
| NCBI Synonym Full Names: | regulator of chromosome condensation 1 |
| NCBI Official Symbol: | RCC1 |
| NCBI Official Synonym Symbols: | CHC1; RCC1-I; SNHG3-RCC1 |
| NCBI Protein Information: | regulator of chromosome condensation; SNHG3-RCC1 readthrough; cell cycle regulatory protein; guanine nucleotide-releasing protein |
| UniProt Protein Name: | Regulator of chromosome condensation |
| UniProt Synonym Protein Names: | Cell cycle regulatory protein; Chromosome condensation protein 1 |
| Protein Family: | RCC1 domain-containing protein |
| UniProt Gene Name: | RCC1 |
| UniProt Entry Name: | RCC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RCC1 antibody (CAB6350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded rat lung using RCC1 antibody (CAB6350) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using RCC1 antibody (CAB6350) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using RCC1 antibody (CAB6350) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using RCC1 antibody (CAB6350) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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