Antibody Name:	Anti-RCAN1 Antibody
Antibody SKU:	CAB5326
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-245 of human RCAN1 (NP_004405.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	SW480, SKOV3, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-245 of human RCAN1 (NP_004405.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DLQD LPSA TIAC HLDP RVFV DGLC RAKF ESLF RTYD KDIT FQYF KSFK RVRI NFSN PFSA ADAR LQLH KTEF LGKE MKLY FAQT LHIG SSHL APPN PDKQ FLIS PPAS PPVG WKQV EDAT PVIN YDLL YAIS KLGP GEKY ELHA ATDT TPSV VVHV CESD QEKE EEEE MERM RRPK PKII QTRR PE
Gene ID:	1827
Uniprot:	P53805
Cellular Location:
Calculated MW:	13kDa/19kDa/22kDa/28kDa
Observed MW:	31kDa

Synonyms:

RCAN1, ADAPT78, CSP1, DSC1, DSCR1, MCIP1, RCN1

Background:

The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	RCAN1: Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development. Interacts with RAF1. By calcium. Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues. Belongs to the RCAN family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 21q22.12 Cellular Component: cytoplasm; nucleus Molecular Function:calcium-dependent protein serine/threonine phosphatase regulator activity; DNA binding; transcription factor activity Biological Process: blood circulation; central nervous system development; signal transduction
NCBI Summary:	The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
UniProt Code:	P53805
NCBI GenInfo Identifier:	215274235
NCBI Gene ID:	1827
NCBI Accession:	P53805.4
UniProt Secondary Accession:	P53805,O00582, O00583, Q53XT0, Q6IBC6, Q7Z555, Q96R03 Q9BU69, Q9UF15, Q9UME4, D3DSF9,
UniProt Related Accession:	P53805
Molecular Weight:	13,291 Da
NCBI Full Name:	Calcipressin-1
NCBI Synonym Full Names:	regulator of calcineurin 1
NCBI Official Symbol:	RCAN1
NCBI Official Synonym Symbols:	CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
NCBI Protein Information:	calcipressin-1
UniProt Protein Name:	Calcipressin-1
UniProt Synonym Protein Names:	Adapt78; Down syndrome critical region protein 1; Myocyte-enriched calcineurin-interacting protein 1; MCIP1; Regulator of calcineurin 1
Protein Family:	Calcipressin
UniProt Gene Name:	RCAN1
UniProt Entry Name:	RCAN1_HUMAN

	Western blot analysis of extracts of various cell lines, using RCAN1 antibody (CAB5326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using RCAN1 antibody (CAB5326). Blue: DAPI for nuclear staining.