Cell Biology Antibodies 6
Anti-RC3H1 Antibody (CAB15569)
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- SKU:
- CAB15569
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RC3H1 Antibody |
| Antibody SKU: | CAB15569 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human RC3H1 (NP_742068.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human RC3H1 (NP_742068.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AAGS VEMM NVES KGMR DQRL DLQR RAAE TSDD DLIP FGDR PTVS RFGA ISRT SKTI YQGA GPMQ AMAP QGAP TKSI NISD YSPY GTHG GWGA SPYS PHQN IPSQ GHFS ERER ISMS EVAS HGKP LPSA EREQ LRLE LQQL NHQI SQQT QLRG LEAV SNRL VLQR EANT LAGQ SQPP PPPP PKWP GMIS SEQL SLEL HQVE REIG KRTR ELSM ENQC SLDM KSKL NTSK QAEN GQPE PQNK VPAE DLTL TFSD VPNG SALT QENI SLLS NKTS SLNL SEDP EGGG DNND SQRS GVTP SSAP |
| Gene ID: | 149041 |
| Uniprot: | Q5TC82 |
| Cellular Location: | Cytoplasm, P-body |
| Calculated MW: | 124kDa/125kDa |
| Observed MW: | 126KDa |
| Synonyms: | RC3H1, RNF198, ROQUIN, roquin-1 |
| Background: | This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RC3H1: Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; RNA-binding Chromosomal Location of Human Ortholog: 1q25.1 Cellular Component: stress granule Molecular Function:mRNA 3'-UTR binding Biological Process: cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; negative regulation of germinal center formation; negative regulation of T-helper cell differentiation; regulation of germinal center formation; regulation of mRNA stability; regulation of T cell receptor signaling pathway |
| NCBI Summary: | This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q5TC82 |
| NCBI GenInfo Identifier: | 73621450 |
| NCBI Gene ID: | 149041 |
| NCBI Accession: | Q5TC82.1 |
| UniProt Secondary Accession: | Q5TC82,Q5W180, Q5W181, Q8IVE6, Q8N9V1, B3KVK1, |
| UniProt Related Accession: | Q5TC82 |
| Molecular Weight: | 124,842 Da |
| NCBI Full Name: | Roquin-1 |
| NCBI Synonym Full Names: | ring finger and CCCH-type domains 1 |
| NCBI Official Symbol: | RC3H1 |
| NCBI Official Synonym Symbols: | RNF198; ROQUIN |
| NCBI Protein Information: | roquin-1 |
| UniProt Protein Name: | Roquin-1 |
| UniProt Synonym Protein Names: | RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198 |
| Protein Family: | Roquin |
| UniProt Gene Name: | RC3H1 |
| UniProt Entry Name: | RC3H1_HUMAN |
 | Western blot analysis of extracts of SH-SY5Y cells, using RC3H1 Rabbit pAb (CAB15569) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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