Name: Anti-RBPMS Antibody (CAB15395)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB15395
Availability: OutOfStock

Antibody Name:	Anti-RBPMS Antibody
Antibody SKU:	CAB15395
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 110-219 of human RBPMS (NP_001008712.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse kidney, Mouse liver, Mouse lung, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 110-219 of human RBPMS (NP_001008712.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVGT PNPS TPLP NTVP QFIA REPY ELTV PALY PSSP EVWA PYPL YPAE LAPA LPPP AFTY PASL HAQC FSPE AKPN TPVF CPLL QQIR FVSG NVFV TYQP TADQ QREL PC
Gene ID:	11030
Uniprot:	Q93062
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	15kDa/17kDa/21kDa/22kDa/24kDa
Observed MW:	22kDa

Synonyms:

RBPMS, HERMES

Background:

This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	RBPMS: Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C- terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 8p12 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding; transcription coactivator activity; nucleotide binding; poly(A) binding Biological Process: RNA processing; regulation of transcription, DNA-dependent; transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
UniProt Code:	Q93062
NCBI GenInfo Identifier:	13124469
NCBI Gene ID:	11030
NCBI Accession:	Q93062.1
UniProt Related Accession:	Q93062
Molecular Weight:	Observed Molecular Weight: (Observed)21kD.Predicted Molecular Weight: (Calculated)22kDa.
NCBI Full Name:	RNA-binding protein with multiple splicing
NCBI Synonym Full Names:	RNA binding protein, mRNA processing factor
NCBI Official Symbol:	RBPMS
NCBI Official Synonym Symbols:	HERMES
NCBI Protein Information:	RNA-binding protein with multiple splicing
UniProt Protein Name:	RNA-binding protein with multiple splicing
UniProt Synonym Protein Names:	Heart and RRM expressed sequence; Hermes
Protein Family:	RNA-binding protein with multiple splicing
UniProt Gene Name:	RBPMS
UniProt Entry Name:	RBPMS_HUMAN

Western blot analysis of extracts of various cell lines, using RBPMS antibody (CAB15395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

RBPMS Antibody (PACO11774)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)