Epigenetics & Nuclear Signaling Antibodies 2

Anti-RBMX Antibody (CAB15436)

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SKU:
CAB15436
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RBMX Antibody
Antibody SKU:CAB15436
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RBMX (NP_002130.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human
Positive Samples:MCF7, COS-7, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RBMX (NP_002130.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVEA DRPG KLFI GGLN TETN EKAL EAVF GKYG RIVE VLLM KDRE TNKS RGFA FVTF ESPA DAKD AARD MNGK SLDG KAIK VEQA TKPS FESG RRG
Gene ID:27316
Uniprot:P38159
Cellular Location:Nucleus
Calculated MW:22kDa/40kDa/42kDa
Observed MW:42kDa
Synonyms:RBMX, HNRNPG, HNRPG, MRXS11, RBMXP1, RBMXRT, RNMX, hnRNP-G, X-linked
Background:This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene, found on chromosomes 1, 4, 9, 11, and 6, were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns.
UniProt Protein Function:RBMX: RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single- stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1- beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA processing; RNA splicing; RNA-binding; Spliceosome; Tumor suppressor

Chromosomal Location of Human Ortholog: Xq26.3

Cellular Component: extracellular space; membrane; nucleoplasm; nucleus

Molecular Function:chromatin binding; identical protein binding; mRNA binding; protein binding; RNA binding

Biological Process: gene expression; membrane protein ectodomain proteolysis; negative regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; osteoblast differentiation; positive regulation of nuclear mRNA splicing, via spliceosome; positive regulation of transcription from RNA polymerase II promoter; protein homooligomerization; regulation of alternative nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter

Disease: Mental Retardation, X-linked, Syndromic 11

NCBI Summary:This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene, found on chromosomes 1, 4, 9, 11, and 6, were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns. [provided by RefSeq, Sep 2009]
UniProt Code:P38159
NCBI GenInfo Identifier:23503093
NCBI Gene ID:27316
NCBI Accession:P38159.3
UniProt Secondary Accession:P38159,Q5JQ67, Q8N8Y7, Q969R3, B4E3U4, D3DWH0, E9PG86
UniProt Related Accession:P38159
Molecular Weight:42kDa
NCBI Full Name:RNA-binding motif protein, X chromosome
NCBI Synonym Full Names:RNA binding motif protein, X-linked
NCBI Official Symbol:RBMX  
NCBI Official Synonym Symbols:RNMX; HNRPG; HNRNPG; MRXS11; RBMXP1; RBMXRT; hnRNP-G  
NCBI Protein Information:RNA-binding motif protein, X chromosome
UniProt Protein Name:RNA-binding motif protein, X chromosome
UniProt Synonym Protein Names:Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G
Protein Family:RNA-binding motif protein
UniProt Gene Name:RBMX  
Anti-RBMX Antibody (CAB15436)Western blot analysis of extracts of various cell lines, using RBMX antibody (CAB15436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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