Cell Death Antibodies 1

Anti-RBM5 Antibody (CAB10788)

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SKU:
CAB10788
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-RBM5 Antibody
Antibody SKU:CAB10788
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBM5 (NP_005769.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, 293T, HeLa, LO2, A-549, Mouse thymus, Mouse liver, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBM5 (NP_005769.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSD KRVS RTER SGRY GSII DRDD RDER ESRS RRRD SDYK RSSD DRRG DRYD DYRD YDSP ERER ERRN SDRS EDGY HSDG DYGE HDYR HDIS DERE SKTI MLRG LPIT ITES DIRE MMES FEGP QPAD VR
Gene ID:10181
Uniprot:P52756
Cellular Location:Nucleus
Calculated MW:14kDa/16kDa/17kDa/92kDa
Observed MW:120kDa
Synonyms:RBM5, G15, H37, LUCA15, RMB5
Background:This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer.
UniProt Protein Function:RBM5: Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. Belongs to the RBM5/RBM10 family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA splicing; C2H2-type zinc finger protein; Apoptosis; Cell cycle regulation; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: nucleoplasm; nucleus; spliceosome

Molecular Function:DNA binding; mRNA binding; protein binding; RNA binding

Biological Process: negative regulation of cell proliferation; nuclear mRNA splicing, via spliceosome; positive regulation of apoptosis; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA processing; spliceosome assembly

NCBI Summary:This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011]
UniProt Code:P52756
NCBI GenInfo Identifier:13124794
NCBI Gene ID:10181
NCBI Accession:P52756.2
UniProt Secondary Accession:P52756,Q93021, Q9BU14, Q9HDA6, Q9UKY8, Q9UL24, B2RA45 B4DM16, B4DMF9, B4DZ63,
UniProt Related Accession:P52756
Molecular Weight:17,891 Da
NCBI Full Name:RNA-binding protein 5
NCBI Synonym Full Names:RNA binding motif protein 5
NCBI Official Symbol:RBM5  
NCBI Official Synonym Symbols:G15; H37; RMB5; LUCA15  
NCBI Protein Information:RNA-binding protein 5
UniProt Protein Name:RNA-binding protein 5
UniProt Synonym Protein Names:Protein G15; Putative tumor suppressor LUCA15; RNA-binding motif protein 5; Renal carcinoma antigen NY-REN-9
Protein Family:RNA-binding protein
UniProt Gene Name:RBM5  
UniProt Entry Name:RBM5_HUMAN
Anti-RBM5 Antibody (CAB10788)Western blot analysis of extracts of various cell lines, using RBM5 antibody (CAB10788) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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