Epigenetics & Nuclear Signaling Antibodies 2
Anti-RBM4B Antibody (CAB14682)
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- SKU:
- CAB14682
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RBM4B Antibody |
| Antibody SKU: | CAB14682 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-359 of human RBM4B (NP_113680.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | LO2, 293T, U-87MG, Raji, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 140-359 of human RBM4B (NP_113680.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RMHV QLST SRLR TAPG MGDQ SGCY RCGK EGHW SKEC PVDR TGRV ADFT EQYN EQYG AVRT PYTM GYGE SMYY NDAY GALD YYKR YRVR SYEA VAAA AAAS AYNY AEQT MSHL PQVQ STTV TSHL NSTS VDPY DRHL LPNS GAAA TSAA MAAA AATT SSYY GRDR SPLR RAAA MLPT VGEG YGYG PESE LSQA SAAT RNSL YDMA RYER EQYV DRAR YSAF |
| Gene ID: | 83759 |
| Uniprot: | Q9BQ04 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 40kDa |
| Observed MW: | 38kDa |
| Synonyms: | RBM4B, RBM30, RBM4L, ZCCHC15, ZCCHC21B, ZCRB3B |
| Background: |
| UniProt Protein Function: | RBM30: Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. |
| UniProt Protein Details: | Protein type:RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 11q13 Cellular Component: nucleolus Molecular Function:protein binding; zinc ion binding; nucleotide binding Biological Process: regulation of translation; circadian rhythm; RNA splicing; circadian regulation of gene expression; mRNA processing; entrainment of circadian clock by photoperiod |
| UniProt Code: | Q9BQ04 |
| NCBI GenInfo Identifier: | 13436323 |
| NCBI Gene ID: | 83759 |
| NCBI Accession: | AAH04951.1 |
| UniProt Secondary Accession: | Q9BQ04,B3KT83, |
| UniProt Related Accession: | Q9BQ04 |
| Molecular Weight: | 40,150 Da |
| NCBI Full Name: | RNA binding motif protein 4B |
| NCBI Synonym Full Names: | RNA binding motif protein 4B |
| NCBI Official Symbol: | RBM4B |
| NCBI Official Synonym Symbols: | RBM30; RBM4L; ZCRB3B; ZCCHC15; ZCCHC21B |
| NCBI Protein Information: | RNA-binding protein 4B; RNA-binding motif protein 30; zinc finger CCHC-type and RNA binding motif 3B |
| UniProt Protein Name: | RNA-binding protein 4B |
| UniProt Synonym Protein Names: | RNA-binding motif protein 30; RNA-binding motif protein 4B; RNA-binding protein 30 |
| Protein Family: | RNA-binding protein |
| UniProt Gene Name: | RBM4B |
| UniProt Entry Name: | RBM4B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RBM4B antibody (CAB14682) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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