Epigenetics & Nuclear Signaling Antibodies 3
Anti-RBM17 Antibody (CAB3240)
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- SKU:
- CAB3240
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RBM17 Antibody |
| Antibody SKU: | CAB3240 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, A-549, HeLa, DU145, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RBM17 (NP_116294.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSLY DDLG VETS DSKT EGWS KNFK LLQS QLQV KKAA LTQA KSQR TKQS TVLA PVID LKRG GSSD DRQI VDTP PHVA AGLK DPVP SGFS AGEV LIPL ADEY DPMF PNDY EKVV KRQR EERQ RQRE LERQ KEIE EREK RRKD RHEA SGFA RRPD PDSD EDED YERE RRKR SMGG AAIA PPTS LVEK DKEL PRDF PYEE DSRP RSQS SKAA IPPP VYEE QDRP RSPT GPSN SFLA NMGG TVAH |
| Gene ID: | 84991 |
| Uniprot: | Q96I25 |
| Cellular Location: | Nucleus |
| Calculated MW: | 44kDa |
| Observed MW: | 50kDa |
| Synonyms: | RBM17, SPF45 |
| Background: | This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. |
| UniProt Protein Function: | RBM17: Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 10p15.1 Cellular Component: nucleoplasm; spliceosome Molecular Function:protein binding; RNA binding; nucleotide binding Biological Process: alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009] |
| UniProt Code: | Q96I25 |
| NCBI GenInfo Identifier: | 34925383 |
| NCBI Gene ID: | 84991 |
| NCBI Accession: | Q96I25.1 |
| UniProt Secondary Accession: | Q96I25,Q96GY6, |
| UniProt Related Accession: | Q96I25 |
| Molecular Weight: | 401 |
| NCBI Full Name: | Splicing factor 45 |
| NCBI Synonym Full Names: | RNA binding motif protein 17 |
| NCBI Official Symbol: | RBM17 |
| NCBI Official Synonym Symbols: | SPF45 |
| NCBI Protein Information: | splicing factor 45; splicing factor 45kDa; 45 kDa-splicing factor; RNA-binding motif protein 17 |
| UniProt Protein Name: | Splicing factor 45 |
| UniProt Synonym Protein Names: | 45 kDa-splicing factor; RNA-binding motif protein 17 |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | RBM17 |
| UniProt Entry Name: | SPF45_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RBM17 antibody (CAB3240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat lung using RBM17 antibody (CAB3240) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human endometrium cancer using RBM17 antibody (CAB3240) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using RBM17 antibody (CAB3240) at dilution of 1:100 (40x lens). |
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