Name: Anti-RBM14 Antibody (CAB13159)
Brand: Immunology Antibodies 1
SKU: CAB13159
Availability: OutOfStock

Antibody Name:	Anti-RBM14 Antibody
Antibody SKU:	CAB13159
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500-600 of human RBM14 (NP_006319.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse thymus, Mouse brain, Mouse eye, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500-600 of human RBM14 (NP_006319.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAAA YGAQ PSAT LAAP YRTQ SSAS LAAS YAAQ QHPQ AAAS YRGQ PGNA YDGA GQPS AAYL SMSQ GAVA NANS TPPP YERT RLSP PRAS YDDP YKKA VAMS K
Gene ID:	10432
Uniprot:	Q96PK6
Cellular Location:	Nucleus, nucleolus
Calculated MW:	12kDa/13kDa/17kDa/37kDa/69kDa
Observed MW:	69kDa

Synonyms:

RBM14, COAA, PSP2, SIP, SYTIP1, TMEM137

Background:

This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene.

UniProt Protein Function:	RBM14: an RNA binding protein and transcriptional regulator. Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. Two alternatively spliced isoforms have been observed. Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1.
UniProt Protein Details:	Protein type:Nucleolus; RNA-binding; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: nucleoplasm; transcription factor complex; nucleolus; Srb-mediator complex; ribonucleoprotein complex Molecular Function:protein binding, bridging; protein binding; ligand-dependent nuclear receptor transcription coactivator activity; RNA binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; estrogen receptor signaling pathway; glucocorticoid receptor signaling pathway; response to hormone stimulus; histone deacetylation; positive regulation of transcription from RNA polymerase II promoter; DNA repair; DNA replication; DNA recombination
NCBI Summary:	This gene encodes a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation. Alternatively spliced transcript variants encoding different isoforms (with opposing effects on transcription) have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	Q96PK6
NCBI GenInfo Identifier:	73913750
NCBI Gene ID:	10432
NCBI Accession:	Q96PK6.2
UniProt Secondary Accession:	Q96PK6,Q8C2Q3,
UniProt Related Accession:	Q96PK6
Molecular Weight:	669
NCBI Full Name:	RNA-binding protein 14
NCBI Synonym Full Names:	RNA binding motif protein 14
NCBI Official Symbol:	RBM14
NCBI Official Synonym Symbols:	SIP; COAA; PSP2; SYTIP1; TMEM137
NCBI Protein Information:	RNA-binding protein 14; paraspeckle protein 2; SYT-interacting protein; transmembrane protein 137; synaptotagmin-interacting protein; RRM-containing coactivator activator/modulator
UniProt Protein Name:	RNA-binding protein 14
UniProt Synonym Protein Names:	Paraspeckle protein 2; PSP2; RNA-binding motif protein 14; RRM-containing coactivator activator/modulator; Synaptotagmin-interacting protein; SYT-interacting protein
Protein Family:	RNA-binding protein
UniProt Gene Name:	RBM14
UniProt Entry Name:	RBM14_HUMAN

Western blot analysis of extracts of various cell lines, using RBM14 antibody (CAB13159) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-RBM14 Antibody (CAB4418)

RBM14 Antibody (PACO11751)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)