Epigenetics & Nuclear Signaling Antibodies 4

Anti-RBFOX1 Antibody (CAB7811)

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SKU:
CAB7811
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RBFOX1 Antibody
Antibody SKU:CAB7811
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBFOX1 (NP_665900.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, Mouse brain, Mouse heart, Mouse skeletal muscle, Rat brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBFOX1 (NP_665900.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLAS QGVL LHPY GVPM IVPA APYL PGLI QGNQ EAAA APDT MAQP YASA QFAP PQNG IPAE YTAP HPHP APEY TGQT TVPE HTLN LYPP AQTH SEQS PADT SAQT VSGT ATQT DDAA PTDG QPQT QPSE NT
Gene ID:54715
Uniprot:Q9NWB1
Cellular Location:Cytoplasm, Nucleus
Calculated MW:40kDa/42kDa/44kDa
Observed MW:43kDa
Synonyms:RBFOX1, 2BP1, A2BP1, FOX-1, FOX1, HRNBP1
Background:The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
NCBI Summary:The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:Q9NWB1
NCBI GenInfo Identifier:215272410
NCBI Gene ID:54715
NCBI Accession:NP_001135805.1
UniProt Secondary Accession:Q9NWB1,Q7Z7I7, Q8TAE3, Q8TAF2, Q8WYB2, Q9NS20,
UniProt Related Accession:Q9NWB1
Molecular Weight:Observed: 43 kDaPredicted: 43 kDa
NCBI Full Name:RNA binding protein fox-1 homolog 1 isoform 5
NCBI Synonym Full Names:RNA binding fox-1 homolog 1
NCBI Official Symbol:RBFOX1  
NCBI Official Synonym Symbols:2BP1; FOX1; A2BP1; FOX-1; HRNBP1  
NCBI Protein Information:RNA binding protein fox-1 homolog 1
UniProt Protein Name:RNA binding protein fox-1 homolog 1
UniProt Synonym Protein Names:Ataxin-2-binding protein 1; Fox-1 homolog A; Hexaribonucleotide-binding protein 1
Protein Family:Berberine bridge enzyme
UniProt Gene Name:RBFOX1  
Anti-RBFOX1 Antibody (CAB7811)Western blot analysis of extracts of various cell lines, using RBFOX1 antibody (CAB7811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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