Epigenetics & Nuclear Signaling Antibodies 4

Anti-RBBP5 Antibody (CAB6965)

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SKU:
CAB6965
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RBBP5 Antibody
Antibody SKU:CAB6965
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 319-538 of human RBBP5 (NP_005048.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, NCI-H460, HeLa, Mouse spleen, Mouse brain, Mouse thymus, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 319-538 of human RBBP5 (NP_005048.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SIWA QNQV ENWS AFAP DFKE LDEN VEYE ERES EFDI EDED KSEP EQTG ADAA EDEE VDVT SVDP IAAF CSSD EELE DSKA LLYL PIAP EVED PEEN PYGP PPDA VQTS LMDE GASS EKKR QSSA DGSQ PPKK KPKT TNIE LQGV PNDE VHPL LGVK GDGK SKKK QAGR PKGS KGKE KDSP FKPK LYKG DRGL PLEG SAKG KVQA ELSQ PLTA GGAI SELL
Gene ID:5929
Uniprot:Q15291
Cellular Location:Nucleus
Calculated MW:55kDa/59kDa
Observed MW:70kDa
Synonyms:RBBP5, RBQ3, SWD1
Background:This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
UniProt Protein Function:RBBP5: As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with WDR5 and ASH2L; the interaction is direct. Interacts with WDR82 and SETD1A. Ubiquitously expressed. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: histone methyltransferase complex; nucleolus; nucleoplasm; nucleus

Molecular Function:histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; methylated histone residue binding; protein binding

Biological Process: histone H3-K4 methylation; response to DNA damage stimulus; response to estrogen stimulus

NCBI Summary:This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2010]
UniProt Code:Q15291
NCBI GenInfo Identifier:209572664
NCBI Gene ID:5929
NCBI Accession:Q15291.2
UniProt Secondary Accession:Q15291,Q7Z6D8, Q8NDZ7, A8K272,
UniProt Related Accession:Q15291
Molecular Weight:55,072 Da
NCBI Full Name:Retinoblastoma-binding protein 5
NCBI Synonym Full Names:RB binding protein 5, histone lysine methyltransferase complex subunit
NCBI Official Symbol:RBBP5  
NCBI Official Synonym Symbols:RBQ3; SWD1  
NCBI Protein Information:retinoblastoma-binding protein 5
UniProt Protein Name:Retinoblastoma-binding protein 5
UniProt Synonym Protein Names:Retinoblastoma-binding protein RBQ-3
Protein Family:Retinoblastoma-binding protein
UniProt Gene Name:RBBP5  
UniProt Entry Name:RBBP5_HUMAN
Anti-RBBP5 Antibody (CAB6965)Western blot analysis of extracts of various cell lines, using RBBP5 antibody (CAB6965) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-RBBP5 Antibody (CAB6965)Immunohistochemistry of paraffin-embedded rat Intestine using RBBP5 antibody (CAB6965) at dilution of 1:100 (40x lens).
Anti-RBBP5 Antibody (CAB6965)Immunohistochemistry of paraffin-embedded human colon carcinoma using RBBP5 antibody (CAB6965) at dilution of 1:100 (40x lens).
Anti-RBBP5 Antibody (CAB6965)Immunohistochemistry of paraffin-embedded mouse liver using RBBP5 antibody (CAB6965) at dilution of 1:100 (40x lens).
Anti-RBBP5 Antibody (CAB6965)Immunofluorescence analysis of A-549 cells using RBBP5 antibody (CAB6965). Blue: DAPI for nuclear staining.
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