Antibody Name:	Anti-RBBP4 Antibody [KO Validated]
Antibody SKU:	CAB13934
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP ChIP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human RBBP4 (NP_005601.1).

Application:	WB IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 ChIP 1:20 - 1:50
Reactivity:	Human
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human RBBP4 (NP_005601.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADK EAAF DDAV EERV INEE YKIW KKNT PFLY DLVM THAL EWPS LTAQ WLPD VTRP EGKD FSIH RLVL GTHT SDEQ NHLV IASV QLPN DDAQ FDAS HYDS EKGE FGGF GSVS GKIE IEIK INHE GEVN RARY MPQN PCII ATKT PSSD VLVF DYTK HPSK PDPS GECN PDLR LRGH QKEG YGLS WNPN LSGH LLSA SDDH TICL WDIS AVPK EGKV VDAK TIFT GHTA VVED VSWH LLHE SLFG SVAD DQKL MIWD TRSN NTSK PSHS VDAH TAEV NCLS FNPY SEFI LATG SADK TVAL WDLR NLKL KLHS FESH KDEI FQVQ WSPH NETI LASS GTDR RLNV WDLS KIGE EQSP EDAE DGPP ELLF IHGG HTAK ISDF SWNP NEPW VICS VSED NIMQ VWQM AENI YNDE DPEG SVDP EGQG S
Gene ID:	5928
Uniprot:	Q09028
Cellular Location:	Nucleus
Calculated MW:	43kDa/46kDa/47kDa
Observed MW:	48KDa

Synonyms:

RBBP4, NURF55, RBAP48, lin-53

Background:

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RBBP4: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. Interacts with SUV39H1 and HDAC7. Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Component of the PRC2/EED- EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p35.1 Cellular Component: nucleoplasm; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; NuRD complex; nucleus Molecular Function:DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; histone binding; histone deacetylase binding Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of cell cycle; chromatin assembly; regulation of gene expression, epigenetic; DNA replication-dependent nucleosome assembly; chromatin remodeling; negative regulation of cell proliferation; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle; DNA replication
NCBI Summary:	This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:	Q09028
NCBI GenInfo Identifier:	1172846
NCBI Gene ID:	5928
NCBI Accession:	Q09028.3
UniProt Secondary Accession:	Q09028,P31149, Q53H02, Q96BV9, B2R6G9, B4DRH0, D3DPQ3
UniProt Related Accession:	Q09028
Molecular Weight:	425
NCBI Full Name:	Histone-binding protein RBBP4
NCBI Synonym Full Names:	retinoblastoma binding protein 4
NCBI Official Symbol:	RBBP4Â Â
NCBI Official Synonym Symbols:	NURF55; RBAP48; lin-53Â Â
NCBI Protein Information:	histone-binding protein RBBP4; RBBP-4; CAF-I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; MSI1 protein homolog; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 sub
UniProt Protein Name:	Histone-binding protein RBBP4
UniProt Synonym Protein Names:	Chromatin assembly factor 1 subunit C; CAF-1 subunit C; Chromatin assembly factor I p48 subunit; CAF-I 48 kDa subunit; CAF-I p48; Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4; RBBP-4; Retinoblastoma-binding protein p48
Protein Family:	Histone-binding protein
UniProt Gene Name:	RBBP4Â Â
UniProt Entry Name:	RBBP4_HUMAN

	Western blot analysis of extracts of 293T cells, using RBBP4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.