Cell Biology Antibodies 6
Anti-RASGRP2 Antibody (CAB15381)
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- SKU:
- CAB15381
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RASGRP2 Antibody |
| Antibody SKU: | CAB15381 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RASGRP2 (NP_722541.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 293T, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RASGRP2 (NP_722541.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGT LDLD KGCT VEEL LRGC IEAF DDSG KVRD PQLV RMFL MMHP WYIP SSQL AAKL LHIY QQSR KDNS NSLQ VKTC HLVR YWIS AFPA EFDL NPEL AEQI KELK ALLD QEGN RRHS SLID IDSV PTYK WKRQ VTQR NPVG QKKR KMSL LFDH LEPM ELAE HLTY LEYR SFCK ILFQ DYHS FVTH GCTV DNPV LERF ISLF |
| Gene ID: | 10235 |
| Uniprot: | Q7LDG7 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Peripheral membrane protein, cytosol, ruffle membrane, synapse, synaptosome |
| Calculated MW: | 16kDa/69kDa/75kDa |
| Observed MW: | 69kDa |
| Synonyms: | RASGRP2, CALDAG-GEFI, CDC25L |
| Background: | The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. |
| NCBI Summary: | The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Four alternatively spliced transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| UniProt Code: | Q7LDG7 |
| NCBI GenInfo Identifier: | 74713056 |
| NCBI Gene ID: | 10235 |
| NCBI Accession: | Q7LDG7.1 |
| UniProt Secondary Accession: | Q7LDG7,O00538, Q9UL65, A6NDC7, |
| UniProt Related Accession: | Q7LDG7 |
| Molecular Weight: | 69,320 Da |
| NCBI Full Name: | RAS guanyl-releasing protein 2 |
| NCBI Synonym Full Names: | RAS guanyl releasing protein 2 |
| NCBI Official Symbol: | RASGRP2 |
| NCBI Official Synonym Symbols: | CDC25L; CALDAG-GEFI |
| NCBI Protein Information: | RAS guanyl-releasing protein 2 |
| UniProt Protein Name: | RAS guanyl-releasing protein 2 |
| UniProt Synonym Protein Names: | Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B3.3 kinase-like protein |
| Protein Family: | RAS guanyl-releasing protein |
| UniProt Gene Name: | RASGRP2 |
| UniProt Entry Name: | GRP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RASGRP2 antibody (CAB15381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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