Antibody Name:	Anti-RASGRF1 Antibody
Antibody SKU:	CAB6964
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RASGRF1 (NP_001139120.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQKG IRLN DGHV ASLG LLAR KDGT RKGY LSKR SSDN TKWQ TKWF ALLQ NLLF YFES DSSS RPSG LYLL EGCV CDRA PSPK PALS AKEP LEKQ HYFT VNFS HENQ KALE LRTE DAKD CDEW VAAI AHAS YRTL ATEH EALM QKYL HLLQ IVET EKTV AKQL RQQI EDGE IEIE RLKA EITS LLKD NERI QS
Gene ID:	5923
Uniprot:	Q13972
Cellular Location:
Calculated MW:	55kDa/143kDa/145kDa
Observed MW:	145kDa

Synonyms:

RASGRF1, CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187, ras-GRF1

Background:

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

UniProt Protein Function:	RASGRF1: a guanine nucleotide exchange factor (GEF). Stimulates the dissociation of GDP from RAS protein. Activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit in the mouse brain,. The signaling pathway mediated by this protein may be important for long-term memory. Two alternatively spliced variants have been reported.
UniProt Protein Details:	Protein type:GEFs, Ras; GEFs Chromosomal Location of Human Ortholog: 15q24.2 Cellular Component: cytosol; growth cone; plasma membrane Molecular Function:guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity Biological Process: MAPKKK cascade; neurite development; positive regulation of GTPase activity; positive regulation of Ras protein signal transduction; regulation of Rac protein signal transduction; regulation of Ras protein signal transduction; regulation of synaptic plasticity; signal transduction
NCBI Summary:	The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
UniProt Code:	Q13972
NCBI GenInfo Identifier:	613504205
NCBI Gene ID:	5923
NCBI Accession:	Q13972.2
UniProt Secondary Accession:	Q13972,Q16027, F8VPA5, H0YKF2, J3KQP9,
UniProt Related Accession:	Q13972
Molecular Weight:	143,218 Da
NCBI Full Name:	Ras-specific guanine nucleotide-releasing factor 1
NCBI Synonym Full Names:	Ras protein specific guanine nucleotide releasing factor 1
NCBI Official Symbol:	RASGRF1
NCBI Official Synonym Symbols:	GNRP; GRF1; CDC25; GRF55; CDC25L; H-GRF55; PP13187; ras-GRF1
NCBI Protein Information:	ras-specific guanine nucleotide-releasing factor 1
UniProt Protein Name:	Ras-specific guanine nucleotide-releasing factor 1
UniProt Synonym Protein Names:	Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25
Protein Family:	Ras-specific guanine nucleotide-releasing factor
UniProt Gene Name:	RASGRF1
UniProt Entry Name:	RGRF1_HUMAN

	Western blot analysis of extracts of mouse brain, using RASGRF1 antibody (CAB6964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using RASGRF1 antibody (CAB6964) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using RASGRF1 antibody (CAB6964). Blue: DAPI for nuclear staining.