Cell Biology Antibodies 12

Anti-RASA4 Antibody (CAB9860)

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SKU:
CAB9860
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RASA4 Antibody
Antibody SKU:CAB9860
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human RASA4 (NP_008920.5).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, 293T, Mouse thymus, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human RASA4 (NP_008920.5).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HLPP TFHA VAFY VMDE DALS RDDV IGKV CLTR DTIA SHPK GFSG WAHL TEVD PDEE VQGE IHLR LEVW PGAR ACRL RCSV LEAR DLAP KDRN GTSD PFVR VRYK GRTR ETSI VKKS CYPR WNET FEFE LQEG AMEA LCVE AWDW DLVS RNDF LGKV VIDV QRLR VVQQ EEGW FRLQ PDQS KSRR HDEG NLGS LQLE VRLR DETV LPSS YYQP LVHL LCHE VKLG MQGP GQL
Gene ID:10156
Uniprot:O43374
Cellular Location:Cell membrane, Cytoplasm, Peripheral membrane protein, cytosol
Calculated MW:85kDa/90kDa
Observed MW:100kDa
Synonyms:RASA4, CAPRI, GAPL
Background:This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene.
NCBI Summary:This gene encodes a member of the GAP1 family of GTPase-activating proteins that suppresses the Ras/mitogen-activated protein kinase pathway in response to Ca(2+). Stimuli that increase intracellular Ca(2+) levels result in the translocation of this protein to the plasma membrane, where it activates Ras GTPase activity. Consequently, Ras is converted from the active GTP-bound state to the inactive GDP-bound state and no longer activates downstream pathways that regulate gene expression, cell growth, and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O43374
NCBI GenInfo Identifier:299829175
NCBI Gene ID:10156
NCBI Accession:NP_001073346
UniProt Related Accession:O43374
Molecular Weight:~ 85, 90kDa
NCBI Full Name:ras GTPase-activating protein 4 isoform 2
NCBI Synonym Full Names:RAS p21 protein activator 4
NCBI Official Symbol:RASA4  
NCBI Official Synonym Symbols:GAPL; CAPRI  
NCBI Protein Information:ras GTPase-activating protein 4
UniProt Protein Name:Ras GTPase-activating protein 4
UniProt Synonym Protein Names:Calcium-promoted Ras inactivator; Ras p21 protein activator 4; RasGAP-activating-like protein 2
Protein Family:Ras GTPase-activating protein
UniProt Gene Name:RASA4  
UniProt Entry Name:RASL2_HUMAN
Anti-RASA4 Polyclonal Antibody (CAB9860)Western blot analysis of extracts of various cell lines, using RASA4 antibody (CAB9860) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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