Antibody Name:	Anti-RARG Antibody
Antibody SKU:	CAB7448
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human RARG (NP_000957.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse lung, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human RARG (NP_000957.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KAHQ ETFP SLCQ LGKY TTNS SADH RVQL DLGL WDKF SELA TKCI IKIV EFAK RLPG FTGL SIAD QITL LKAA CLDI LMLR ICTR YTPE QDTM TFSD GLTL NRTQ MHNA GFGP LTDL VFAF AGQL LPLE MDDT ETGL LSAI CLIC GDRM DLEE PEKV DKLQ EPLL EALR LYAR RRRP SQPY MFPR MLMK ITDL RGIS TKGA ERAI TLKM EIPG PMPP LIRE MLEN PEMF EDDS SQPG PHPN ASSE DEVP GGQG KGGL KSPA
Gene ID:	5916
Uniprot:	P13631
Cellular Location:	Nucleus
Calculated MW:	42kDa/47kDa/49kDa/50kDa
Observed MW:	55KDa

Synonyms:

RARG, NR1B3, RARC

Background:

This gene encodes a retinoic acid receptor that belongs to the nuclear hormone receptor family. Retinoic acid receptors (RARs) act as ligand-dependent transcriptional regulators. When bound to ligands, RARs activate transcription by binding as heterodimers to the retinoic acid response elements (RARE) found in the promoter regions of the target genes. In their unbound form, RARs repress transcription of their target genes. RARs are involved in various biological processes, including limb bud development, skeletal growth, and matrix homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RARG: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function. Homodimer. Heterodimer with a RXR molecule. Binds DNA preferentially as a RAR/RXR heterodimer. Forms a complex with PUS1 and the SRA1 RNA in the nucleus. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Nuclear receptor Chromosomal Location of Human Ortholog: 12q13 Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; integral to membrane; nucleus Molecular Function:retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; steroid hormone receptor activity; retinoic acid receptor activity; transcription factor activity Biological Process: retinoic acid receptor signaling pathway; transcription initiation from RNA polymerase II promoter; response to retinoic acid; regulation of myelination; glandular epithelial cell development; embryonic eye morphogenesis; positive regulation of apoptosis; multicellular organism growth; negative regulation of chondrocyte differentiation; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; embryonic camera-type eye development; anterior/posterior pattern formation; negative regulation of cell proliferation; positive regulation of programmed cell death; positive regulation of cell proliferation; neural tube closure; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; transmembrane transport; regulation of cell size; negative regulation of apoptosis
NCBI Summary:	This gene encodes a retinoic acid receptor that belongs to the nuclear hormone receptor family. Retinoic acid receptors (RARs) act as ligand-dependent transcriptional regulators. When bound to ligands, RARs activate transcription by binding as heterodimers to the retinoic acid response elements (RARE) found in the promoter regions of the target genes. In their unbound form, RARs repress transcription of their target genes. RARs are involved in various biological processes, including limb bud development, skeletal growth, and matrix homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	P13631
NCBI GenInfo Identifier:	133498
NCBI Gene ID:	5916
NCBI Accession:	P13631.1
UniProt Secondary Accession:	P13631,P22932, Q15281, Q52LZ8, Q9BYX8, Q9H1I3, Q9UJ38 B7Z492, B7Z4F1, B7ZAE4, J3KNP6,
UniProt Related Accession:	P13631
Molecular Weight:	454
NCBI Full Name:	Retinoic acid receptor gamma
NCBI Synonym Full Names:	retinoic acid receptor, gamma
NCBI Official Symbol:	RARG
NCBI Official Synonym Symbols:	RARC; NR1B3
NCBI Protein Information:	retinoic acid receptor gamma; RAR-gamma; nuclear receptor subfamily 1 group B member 3; retinoic acid nuclear receptor gamma variant 1; retinoic acid nuclear receptor gamma variant 2
UniProt Protein Name:	Retinoic acid receptor gamma
UniProt Synonym Protein Names:	Nuclear receptor subfamily 1 group B member 3
Protein Family:	Retinoic acid receptor
UniProt Gene Name:	RARG
UniProt Entry Name:	RARG_HUMAN

	Western blot analysis of extracts of various cell lines, using RARG antibody (CAB7448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of A549 cells using RARG antibody (CAB7448). Blue: DAPI for nuclear staining.