Cell Biology Antibodies 6
Anti-RAPH1 Antibody (CAB15506)
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- SKU:
- CAB15506
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RAPH1 Antibody |
| Antibody SKU: | CAB15506 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RAPH1 (NP_998754.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, HeLa, HepG2, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RAPH1 (NP_998754.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEQL SDEE IDHG AEED SDKE DQDL DKMF GAWL GELD KLTQ SLDS DKPM EPVK RSPL RQET NMAN FSYR FSIY NLNE ALNQ GETV DLDA LMAD LCSI EQEL SSIG SGNS KRQI TETK ATQK LPVS RHTL KHGT LKGL SSSS NRIA KPSH ASYS LDDV TAQL EQAS LSMD EAAQ QSVL EDTK PLVT NQHR RTAS AGTV SDAE VHSI SNSS HSSI TSAA SSMD SLDI DKVT RPQE LDLT HQGQ |
| Gene ID: | 65059 |
| Uniprot: | Q70E73 |
| Cellular Location: | Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, filopodium, lamellipodium |
| Calculated MW: | 66kDa/67kDa/69kDa/70kDa/72kDa/73kDa/135kDa |
| Observed MW: | 200kDa |
| Synonyms: | RAPH1, ALS2CR18, ALS2CR9, LPD, PREL-2, PREL2, RMO1, RalGDS/AF-6 |
| Background: | This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion. |
| NCBI Summary: | This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q70E73 |
| NCBI GenInfo Identifier: | 42740893 |
| NCBI Gene ID: | 65059 |
| NCBI Accession: | NP_976241.1 |
| UniProt Secondary Accession: | Q70E73,Q96Q37, Q9C0I2, |
| UniProt Related Accession: | Q70E73 |
| Molecular Weight: | 135 kDa |
| NCBI Full Name: | ras-associated and pleckstrin homology domains-containing protein 1 isoform 3 |
| NCBI Synonym Full Names: | Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 |
| NCBI Official Symbol: | RAPH1 |
| NCBI Official Synonym Symbols: | LPD; RMO1; PREL2; PREL-2; ALS2CR9; ALS2CR18; RalGDS/AF-6 |
| NCBI Protein Information: | ras-associated and pleckstrin homology domains-containing protein 1 |
| UniProt Protein Name: | Ras-associated and pleckstrin homology domains-containing protein 1 |
| UniProt Synonym Protein Names: | Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; Lamellipodin; Proline-rich EVH1 ligand 2; PREL-2; Protein RMO1 |
| Protein Family: | Ras-associated and pleckstrin homology domains-containing protein |
| UniProt Gene Name: | RAPH1 |
 | Western blot analysis of extracts of various cell lines, using RAPH1 antibody (CAB15506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using RAPH1 antibody (CAB15506) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using RAPH1 antibody (CAB15506) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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