Name: Anti-RAP1GAP Antibody (CAB10539)
Brand: Cell Biology Antibodies 2
SKU: CAB10539
Availability: OutOfStock

Antibody Name:	Anti-RAP1GAP Antibody
Antibody SKU:	CAB10539
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human RAP1GAP (NP_001139130.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, 293T, SGC-7901, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human RAP1GAP (NP_001139130.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AMGL SNKK PNTV STSH SGSF APNN PDLA KAAG ISLI VPGK SPTR KKSG PFGS RRSS AIGI ENIQ EVQE KRES PPAG QKTP DSGH VSQE PKSE NSST QSSP EMPT TKNR AETA AQRA EALK DFSR SSSS ASSF ASVV EETE GVDG EDTG LESV SSSG TPHK RDSF IYST WLED SVST TSGG SSPG PSRS PHPD AGKL GDPA CPEI KIQL EASE QHMP QLGC
Gene ID:	5909
Uniprot:	P47736
Cellular Location:	Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:	73kDa/75kDa/76kDa/80kDa
Observed MW:	90kDa

Synonyms:

RAP1GAP, RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP

Background:

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins.

UniProt Protein Function:	RAP1GAP: a GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
UniProt Protein Details:	Protein type:GAP; GAP, Ras; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p36.12 Cellular Component: cytosol; membrane Molecular Function:GTPase activator activity; protein binding; protein homodimerization activity; Ras GTPase binding Biological Process: axon guidance; positive regulation of GTPase activity; regulation of GTPase activity
NCBI Summary:	This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]
UniProt Code:	P47736
NCBI GenInfo Identifier:	215273877
NCBI Gene ID:	5909
NCBI Accession:	P47736.2
UniProt Secondary Accession:	P47736,O75062, Q5T3S9, Q5T3T4, Q7Z5S8, Q9UQ51, J3QSS6
UniProt Related Accession:	P47736
Molecular Weight:	73kDa
NCBI Full Name:	Rap1 GTPase-activating protein 1
NCBI Synonym Full Names:	RAP1 GTPase activating protein
NCBI Official Symbol:	RAP1GAP
NCBI Official Synonym Symbols:	RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII
NCBI Protein Information:	rap1 GTPase-activating protein 1
UniProt Protein Name:	Rap1 GTPase-activating protein 1
Protein Family:	Rap1 GTPase-activating protein
UniProt Gene Name:	RAP1GAP

Western blot analysis of extracts of various cell lines, using RAP1GAP antibody (CAB10539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

RAP1GAP Antibody (PACO06794)

RAP1GAP Antibody (PACO11717)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)