Cell Biology Antibodies 8
Anti-RAP1B Antibody (CAB2665)
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- SKU:
- CAB2665
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RAP1B Antibody |
| Antibody SKU: | CAB2665 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human RAP1B |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:1000 IHC 1:50- 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2 |
| Immunogen: | Recombinant protein of human RAP1B |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5908 |
| Uniprot: | P61224 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasm, cytosol |
| Calculated MW: | 15kDa/16kDa/18kDa/20kDa |
| Observed MW: | 21kDa |
| Synonyms: | RAP1B, K-REV, RAL1B |
| Background: | This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. |
| UniProt Protein Function: | RAP1B: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Activated by binding to the GTPase-activating protein RAP1GAP. Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner. Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 12q14 Cellular Component: membrane; plasma membrane; lipid particle; intracellular; intercellular junction; cytosol; lipid raft Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; protein complex binding Biological Process: platelet activation; cell proliferation; microvillus biogenesis; negative regulation of calcium ion-dependent exocytosis; metabolic process; blood coagulation; Rap protein signal transduction |
| NCBI Summary: | This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P61224 |
| NCBI GenInfo Identifier: | 47117723 |
| NCBI Gene ID: | 5908 |
| NCBI Accession: | P61224.1 |
| UniProt Related Accession: | P61224 |
| Molecular Weight: | |
| NCBI Full Name: | Ras-related protein Rap-1b |
| NCBI Synonym Full Names: | RAP1B, member of RAS oncogene family |
| NCBI Official Symbol: | RAP1B |
| NCBI Official Synonym Symbols: | K-REV; RAL1B |
| NCBI Protein Information: | ras-related protein Rap-1b |
| UniProt Protein Name: | Ras-related protein Rap-1b |
| UniProt Synonym Protein Names: | GTP-binding protein smg p21B |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAP1B |
| UniProt Entry Name: | RAP1B_HUMAN |
 | Western blot analysis of extracts of HepG2 cells, using RAP1B antibody (CAB2665). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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