Antibody Name:	Anti-RAP1B Antibody
Antibody SKU:	CAB12925
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RAP1B (NP_056461.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, HeLa, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RAP1B (NP_056461.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MREY KLVV LGSG GVGK SALT VQFV QGIF VEKY DPTI EDSY RKQV EVDA QQCM LEIL DTAG TEQF TAMR DLYM KNGQ GFAL VYSI TAQS TFND LQDL REQI LRVK DTDD VPMI LVGN KCDL EDER VVGK EQGQ NLAR QWNN CAFL ESSA KSKI NVNE IFYD LVRQ INRK TPVP GKAR KKSS CQLL
Gene ID:	5908
Uniprot:	P61224
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, cytosol
Calculated MW:	15kDa/16kDa/18kDa/20kDa
Observed MW:	21kDa

Synonyms:

RAP1B, K-REV, RAL1B

Background:

This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.

UniProt Protein Function:	RAP1B: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Activated by binding to the GTPase-activating protein RAP1GAP. Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner. Belongs to the small GTPase superfamily. Ras family.
UniProt Protein Details:	Protein type:G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 12q14 Cellular Component: membrane; plasma membrane; lipid particle; intracellular; intercellular junction; cytosol; lipid raft Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; protein complex binding Biological Process: platelet activation; cell proliferation; microvillus biogenesis; negative regulation of calcium ion-dependent exocytosis; metabolic process; blood coagulation; Rap protein signal transduction
NCBI Summary:	This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011]
UniProt Code:	P61224
NCBI GenInfo Identifier:	47117723
NCBI Gene ID:	5908
NCBI Accession:	P61224.1
UniProt Related Accession:	P61224
Molecular Weight:
NCBI Full Name:	Ras-related protein Rap-1b
NCBI Synonym Full Names:	RAP1B, member of RAS oncogene family
NCBI Official Symbol:	RAP1B
NCBI Official Synonym Symbols:	K-REV; RAL1B
NCBI Protein Information:	ras-related protein Rap-1b
UniProt Protein Name:	Ras-related protein Rap-1b
UniProt Synonym Protein Names:	GTP-binding protein smg p21B
Protein Family:	Ras-related protein
UniProt Gene Name:	RAP1B
UniProt Entry Name:	RAP1B_HUMAN

	Western blot analysis of extracts of various cell lines, using RAP1B antibody (CAB12925) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using RAP1B Rabbit pAb (CAB12925) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.