Cell Biology Antibodies 1

Anti-RANBP3 Antibody (CAB0955)

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SKU:
CAB0955
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RANBP3 Antibody
Antibody SKU:CAB0955
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RANBP3 (NP_015559.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, 293T, LO2, HT-29, SW620, U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human RANBP3 (NP_015559.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADL ANEE KPAI APPV FVFQ KDKG QKRS AGGS SPEG GEDS DRED GNYC PPVK RERT SSLT QFPP SQSE ERSS GFRL KPPT LIHG QAPS AGLP SQKP KEQQ RSVL RPAV LQAP QPKA LSQT VPSS GTNG VSLP ADCT GAVP AASP DTAA WRSP SEAA DEVC ALEE KEPQ KNES SNAS EEEA CEKK DPAT QQAF VFGQ NLRD RVKL INES VDEA DMEN AGHP SADT PTAT NYFL QYIS SSLE NSTN SADA SS
Gene ID:8498
Uniprot:Q9H6Z4
Cellular Location:Cytoplasm, Nucleus
Calculated MW:53kDa/59kDa/60kDa
Observed MW:75kDa
Synonyms:RANBP3
Background:This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UniProt Protein Function:RANBP3: Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF- beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export. Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling. Widely expressed with high levels in testis and heart. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: positive regulation of protein export from nucleus

NCBI Summary:This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H6Z4
NCBI GenInfo Identifier:51316528
NCBI Gene ID:8498
NCBI Accession:Q9H6Z4.1
UniProt Secondary Accession:Q9H6Z4,O60405, O75759, O75760, Q9BT47, Q9UG74, B2RAT8
UniProt Related Accession:Q9H6Z4
Molecular Weight:53,411 Da
NCBI Full Name:Ran-binding protein 3
NCBI Synonym Full Names:RAN binding protein 3
NCBI Official Symbol:RANBP3  
NCBI Protein Information:ran-binding protein 3
UniProt Protein Name:Ran-binding protein 3
Protein Family:Ran-binding protein
UniProt Gene Name:RANBP3  
UniProt Entry Name:RANB3_HUMAN
Anti-RANBP3 Antibody (CAB0955)Western blot analysis of extracts of various cell lines, using RANBP3 antibody (CAB0955) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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