Cell Biology Antibodies 7
Anti-RANBP1 Antibody (CAB16422)
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- SKU:
- CAB16422
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RANBP1 Antibody |
| Antibody SKU: | CAB16422 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RANBP1 (NP_002873.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, HepG2, Mouse testis, Mouse spleen, Rat testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RANBP1 (NP_002873.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA KDTH EDHD TSTE NTDE SNHD PQFE PIVS LPEQ EIKT LEED EEEL FKMR AKLF RFAS ENDL PEWK ERGT GDVK LLKH KEKG AIRL LMRR DKTL KICA |
| Gene ID: | 5902 |
| Uniprot: | P43487 |
| Cellular Location: | |
| Calculated MW: | 23kDa |
| Observed MW: | 27kDa |
| Synonyms: | RANBP1, HTF9A |
| Background: | This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RANBP1: a Ran-binding protein that functions in nuclear trafficking, mitosis, chromosomal segregation, and ciliogenesis. Controls both nuclear import and export. Contains a Ran binding domain and a C-terminal nuclear export signal. Following export from the nucleus, forms a complex with RanGTP and XPO1/cargo, leading to dissociation of cargo from XPO1. Stimulates RanGAP1 association with RanGTP, facilitating RanGTP hydrolysis to RanGDP. Stabilizes and regulates the formation of a RanGDP-importin/NLS receptor-RanBP1 complex during nuclear import. Controls RanGTP distribution along mitotic microtubules, which localizes critical factors, such as cyclin B1 and DLG7, to mitotic microtubles and regulates chromosome segregation. Knock down or overexpression experiments affect cellular ciliogenesis by regulating the local RanGTP concentation at the base of cilia.Belongs to the RANBP1 family. |
| UniProt Protein Details: | Protein type:G protein regulator, misc.; Microtubule-binding; Cell cycle regulation Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytoplasm; nuclear envelope; nucleus Molecular Function:GDP-dissociation inhibitor activity; Ran GTPase binding; GTPase activator activity Biological Process: intracellular transport; viral reproduction; regulation of catalytic activity; signal transduction; positive regulation of GTPase activity |
| NCBI Summary: | This gene encodes a protein that forms a complex with Ras-related nuclear protein (Ran) and metabolizes guanoside triphosphate (GTP). This complex participates in the regulation of the cell cycle by controlling transport of proteins and nucleic acids into the nucleus. There are multiple pseudogenes for this gene on chromosomes 9, 12, 17, and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P43487 |
| NCBI GenInfo Identifier: | 520975477 |
| NCBI Gene ID: | 5902 |
| NCBI Accession: | NP_001265568.1 |
| UniProt Related Accession: | P43487 |
| Molecular Weight: | |
| NCBI Full Name: | ran-specific GTPase-activating protein isoform 1 |
| NCBI Synonym Full Names: | RAN binding protein 1 |
| NCBI Official Symbol: | RANBP1 |
| NCBI Official Synonym Symbols: | HTF9A |
| NCBI Protein Information: | ran-specific GTPase-activating protein |
| UniProt Protein Name: | Ran-specific GTPase-activating protein |
| UniProt Synonym Protein Names: | Ran-binding protein 1; RanBP1 |
| UniProt Gene Name: | RANBP1 |
| UniProt Entry Name: | RANG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RANBP1 Rabbit pAb (CAB16422) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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