Name: Anti-RAMP2 Antibody (CAB3075)
Brand: Signal Transduction Antibodies 2
SKU: CAB3075
Availability: OutOfStock

Antibody Name:	Anti-RAMP2 Antibody
Antibody SKU:	CAB3075
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human RAMP2 (NP_005845.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	A-549, BxPC-3, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human RAMP2 (NP_005845.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASL RVER AGGP RLPR TRVG RPAA LRLL LLLG AVLN PHEA LAQP LPTT GTPG SEGG TVKN YETA VQFC WNHY KDQM DPIE KDWC DWAM ISRP YSTL RDCL EHFA ELFD LGFP NPLA ERII FETH QIHF ANCS LVQP TFSD PPED V
Gene ID:	10266
Uniprot:	O60895
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	19kDa/20kDa
Observed MW:	15kDa

Synonyms:

RAMP2

Background:

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface.

UniProt Protein Function:	RAMP2: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL. Belongs to the RAMP family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 17q12-q21.1 Cellular Component: cell surface; integral to plasma membrane; lysosome; cytoplasm; plasma membrane; coated pit; receptor complex Molecular Function:protein binding; coreceptor activity; protein transporter activity Biological Process: heart development; female pregnancy; response to estradiol stimulus; cellular response to hormone stimulus; intracellular protein transport; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; protein transport; receptor internalization; calcium ion transport; regulation of blood pressure; positive regulation of cAMP biosynthetic process; response to hypoxia; negative regulation of vascular permeability; cAMP biosynthetic process; angiogenesis; sprouting angiogenesis; vasculogenesis; response to progesterone stimulus; regulation of G-protein coupled receptor protein signaling pathway
NCBI Summary:	The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq, Jul 2008]
UniProt Code:	O60895
NCBI GenInfo Identifier:	27735250
NCBI Gene ID:	10266
NCBI Accession:	O60895.2
UniProt Related Accession:	O60895
Molecular Weight:	~20kDa
NCBI Full Name:	Receptor activity-modifying protein 2
NCBI Synonym Full Names:	receptor activity modifying protein 2
NCBI Official Symbol:	RAMP2
NCBI Protein Information:	receptor activity-modifying protein 2
UniProt Protein Name:	Receptor activity-modifying protein 2
UniProt Synonym Protein Names:	Calcitonin-receptor-like receptor activity-modifying protein 2; CRLR activity-modifying protein 2
Protein Family:	Receptor activity-modifying protein
UniProt Gene Name:	RAMP2
UniProt Entry Name:	RAMP2_HUMAN

Western blot analysis of extracts of various cell lines, using RAMP2 antibody (CAB3075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-RAMP2 Antibody (CAB17072)

RAMP2 Antibody (PACO11705)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)